Evidence map›Paper›PMID 24373284›Full record

ArticleCell reports2013

Hypomethylation of the IL17RC promoter in peripheral blood leukocytes is not a hallmark of age-related macular degeneration.

Verity F Oliver, Maria Franchina, Andrew E Jaffe, Kari E Branham, Mohammad Othman, John R Heckenlively, Anand Swaroop, Betsy Campochiaro, Brendan J Vote, Jamie E Craig and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Review
  3. New perspectives on DNA methylation modifications in ocular diseases.International journal of ophthalmology · 2025
    Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Immunological Aspects of Age-Related Macular Degeneration.Advances in experimental medicine and biology · 2021
    Article
  12. Age-Related Macular Degeneration: From Epigenetics to Therapeutic Implications.Advances in experimental medicine and biology · 2021
    Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 12 institutions in 3 countries.

Verity F OliverDepartment of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Maria FranchinaCentre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth, WA 6009, Australia.
Andrew E JaffeLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21287, USA.
Kari E BranhamKellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
Mohammad OthmanKellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
John R HeckenlivelyKellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
Anand SwaroopNational Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Betsy CampochiaroDepartment of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Brendan J VoteLaunceston Eye Institute, University of Tasmania, Launceston 7249, Australia.
Jamie E CraigDepartment of Ophthalmology, Flinders University, Flinders Medical Centre, Adelaide, SA 5042, Australia.
Richard SafferyCancer and Disease Epigenetics, Murdoch Childrens Research Institute, University of Melbourne, Royal Children's Hospital, Melbourne, VIC 3052, Australia.
David A MackeyCentre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth, WA 6009, Australia.
Jiang QianDepartment of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Donald J ZackDepartment of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA; Department of Molecular Biology and Genetics, Department of Neuroscience, and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA; Institut de la Vision, Université Pierre et Marie Curie, Paris 75012, France.
Alex W HewittCentre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth, WA 6009, Australia; Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia. Electronic address: hewitt.alex@gmail.com.
Shannath L MerbsDepartment of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA. Electronic address: smerbs@jhmi.edu.
Johns Hopkins University · USUniversity of Michigan–Ann Arbor · USFlinders Medical Centre · AUInstitut de la Vision · FRJohns Hopkins Medicine · USLaunceston General Hospital · AULieber Institute for Brain Development · USLions Eye Institute · AUNational Institutes of Health · USRoyal Children's Hospital · AUUniversity of Melbourne · AUUniversity of Western Australia · AU

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Jiang Qian · 1985 to 2026
$22.1M
Genetic susceptibility and common multi-factorial retinal/macular diseasesZIAEY000475 · NEI · NATIONAL EYE INSTITUTE · PI SWAROOP, ANAND · 2009 to 2014
$6.9M
Pangenomic Analysis of DNA Methylation Marks in Glaucoma and Macular DegenerationR01EY020406 · NEI · JOHNS HOPKINS UNIVERSITY · PI MERBS, SHANNATH L · 2009 to 2013
$2.3M
Genetic Variations in Age-related Macular DegenerationR01EY016862 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HECKENLIVELY, JOHN R · 2006 to 2010
$2.1M
NEI NIH HHS EY016862NEI NIH HHS P30 EY001765NEI NIH HHS P30EY001765NEI NIH HHS R01 EY016862NEI NIH HHS R01 EY020406NEI NIH HHS R01EY020406NHGRI NIH HHS X01HG006605
6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is a leading cause of visual impairment worldwide. Aberrant DNA methylation within the promoter of IL17RC in peripheral blood mononuclear cells has recently been reported in AMD. To validate this association, we examined DNA methylation of the IL17RC promoter in peripheral blood. First, we used Illumina Human Methylation450 Bead Arrays, a widely accepted platform for measuring global DNA methylation. Second, methylation status at multiple sites within the IL17RC promoter was determined by bisulfite pyrosequencing in two cohorts. Third, a methylation-sensitive quantitative PCR-based assay was performed on a subset of samples. In contrast to previous findings, we did not find evidence of differential methylation between AMD cases and age-matched controls. We conclude that hypomethylation within the IL17RC gene promoter in peripheral blood is not suitable for use as a clinical biomarker of AMD. This study highlights the need for considerable replication of epigenetic association studies prior to clinical application.

Indexed as

DNA MethylationPromoter Regions, GeneticAgedAged, 80 and overBiomarkersCase-Control StudiesFemaleHumansLeukocytesMacular DegenerationMaleMiddle AgedReceptors, InterleukinBiomarkersIL17RC protein, humanReceptors, Interleukin

Identifiers

PMID24373284
PMCPMC3926096
OpenAlexW2100631250

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.