ArticleBMC biochemistry2013
Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii.
Article in BMC biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 112 citations in OpenAlex.
- Effects of theFrontiers in plant science · 2025Article
- Effect of marker-free transgenic Chlamydomonas on the control of Aedes mosquito population and on plankton.Parasites & vectors · 2023Article
- Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton.PLoS neglected tropical diseases · 2023Article
- Increased Lipids inInternational journal of molecular sciences · 2022Article
- Culture Conditions Affect Antioxidant Production, Metabolism and Related Biomarkers of the MicroalgaeAntioxidants (Basel, Switzerland) · 2022Article
- Development of an RNAi-based microalgal larvicide for the control of Aedes aegypti.Parasites & vectors · 2021Article
- Function of ORFC of the polyketide synthase gene cluster on fatty acid accumulation in Schizochytrium limacinum SR21.Biotechnology for biofuels · 2021Article
- Current perspectives on integrated approaches to enhance lipid accumulation in microalgae.3 Biotech · 2021Review
- Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources.Biotechnology for biofuels · 2021Article
- Molecular Characterization of a Tolerant Saline-AlkaliFrontiers in microbiology · 2021Article
- Controlling the development of the dengue vector Aedes aegypti using HR3 RNAi transgenic Chlamydomonas.PloS one · 2020Article
- Biomass and lipid induction strategies in microalgae for biofuel production and other applications.Microbial cell factories · 2019Review
- Understanding the functions of endogenous DOF transcript factor inBiotechnology for biofuels · 2019Article
- RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina.Scientific reports · 2017Article
- Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.Scientific reports · 2016Article
- Metabolic regulation of triacylglycerol accumulation in the green algae: identification of potential targets for engineering to improve oil yield.Plant biotechnology journal · 2016Review
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe regulation of lipid biosynthesis is essential in photosynthetic eukaryotic cells. This regulation occurs during the direct synthesis of fatty acids and triacylglycerols (TAGs), as well as during other controlling processes in the main carbon metabolic pathway.
resultsIn this study, the mRNA levels of Chlamydomonas citrate synthase (CrCIS) were found to decrease under nitrogen-limited conditions, which suggests suppressed gene expression. Gene silencing by RNA interference (RNAi) was conducted to determine whether CrCIS suppression affected the carbon flux in TAG biosynthesis. Results showed that the TAG level increased by 169.5%, whereas the CrCIS activities in the corresponding transgenic algae decreased by 16.7% to 37.7%. Moreover, the decrease in CrCIS expression led to the increased expression of TAG biosynthesis-related genes, such as acyl-CoA:diacylglycerol acyltransferase and phosphatidate phosphatase. Conversely, overexpression of CrCIS gene decreased the TAG level by 45% but increased CrCIS activity by 209% to 266% in transgenic algae.
conclusionsThe regulation of CrCIS gene can indirectly control the lipid content of algal cells. Our findings propose that increasing oil by suppressing CrCIS expression in microalgae is feasible.
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