ArticleMolecular microbiology2014
Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Enteric-colonizedGut microbes · 2026Article
- Gut microbiota-derived hexa-acylated lipopolysaccharides enhance cancer immunotherapy responses.Nature microbiology · 2025Article
- Article
- Variation, Modification and Engineering of Lipid A in Endotoxin of Gram-Negative Bacteria.International journal of molecular sciences · 2021Review
- Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules.Chemical reviews · 2020Review
- Pushing the envelope: LPS modifications and their consequences.Nature reviews. Microbiology · 2019Review
- Rickettsia Lipid A Biosynthesis Utilizes the Late Acyltransferase LpxJ for Secondary Fatty Acid Addition.Journal of bacteriology · 2018Article
- Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.International journal of microbiology · 2018Review
- AlmG, responsible for polymyxin resistance in pandemicThe Journal of biological chemistry · 2017Article
- Helicobacter pylori modulates host cell responses by CagT4SS-dependent translocation of an intermediate metabolite of LPS inner core heptose biosynthesis.PLoS pathogens · 2017Article
- Characterization of Lipid A Variants by Energy-Resolved Mass Spectrometry: Impact of Acyl Chains.Journal of the American Society for Mass Spectrometry · 2017Article
- The Power of Asymmetry: Architecture and Assembly of the Gram-Negative Outer Membrane Lipid Bilayer.Annual review of microbiology · 2016Review
- UVliPiD: A UVPD-Based Hierarchical Approach for De Novo Characterization of Lipid A Structures.Analytical chemistry · 2016Article
- Article
- Helicobacter pylori: expect the unexpected.Molecular microbiology · 2014Article
- Progress in Characterization of Lipopolysaccharides and Lipid A by Mass Spectrometry.Mass spectrometry reviewsReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Most Gram-negative organisms produce lipopolysaccharide (LPS), a complex macromolecule anchored to the bacterial membrane by the lipid A moiety. Lipid A is synthesized via the Raetz pathway, a conserved nine-step enzymatic process first characterized in Escherichia coli. The Epsilonproteobacterium Helicobacter pylori uses the Raetz pathway to synthesize lipid A; however, only eight of nine enzymes in the pathway have been identified in this organism. Here, we identify the missing acyltransferase, Jhp0255, which transfers a secondary acyl chain to the 3'-linked primary acyl chain of lipid A, an activity similar to that of E. coli LpxM. This enzyme, reannotated as LpxJ due to limited sequence similarity with LpxM, catalyses addition of a C12:0 or C14:0 acyl chain to the 3'-linked primary acyl chain of lipid A, complementing an E. coli LpxM mutant. Enzymatic assays demonstrate that LpxJ and homologues in Campylobacter jejuni and Wolinella succinogenes can act before the 2' secondary acyltransferase, LpxL, as well as the 3-deoxy-d-manno-octulosonic acid (Kdo) transferase, KdtA. Ultimately, LpxJ is one member of a large class of acyltransferases found in a diverse range of organisms that lack an E. coli LpxM homologue, suggesting that LpxJ participates in lipid A biosynthesis in place of an LpxM homologue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.