ArticleProceedings of SPIE--the International Society for Optical Engineering2013
Single-molecule FRET experiments with a red-enhanced custom technology SPAD.
Article in Proceedings of SPIE--the International Society for Optical Engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
- Silicon technologies for arrays of Single Photon Avalanche Diodes.Proceedings of SPIE--the International Society for Optical Engineering · 2016Article
- Silicon photon-counting avalanche diodes for single-molecule fluorescence spectroscopy.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society · 2014Article
- New silicon technologies enable high-performance arrays of Single Photon Avalanche Diodes.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- A 48-pixel array of Single Photon Avalanche Diodes for multispot Single Molecule analysis.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Single-molecule fluorescence spectroscopy of freely diffusing molecules in solution is a powerful tool used to investigate the properties of individual molecules. Single-Photon Avalanche Diodes (SPADs) are the detectors of choice for these applications. Recently a new type of SPAD detector was introduced, dubbed red-enhanced SPAD (RE-SPAD), with good sensitivity throughout the visible spectrum and with excellent timing performance. We report a characterization of this new detector for single-molecule fluorescence resonant energy transfer (smFRET) studies on freely diffusing molecules in a confocal geometry and alternating laser excitation (ALEX) scheme. We use a series of doubly-labeled DNA molecules with donor-to-acceptor distances covering the whole range of useful FRET values. Both intensity-based (μs-ALEX) and lifetime-based (ns-ALEX) measurements are presented and compared to identical measurements performed with standard thick SPADs. Our results demonstrate the great potential of this new detector for smFRET measurements and beyond.
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