ArticleNucleic acids research2014
Targeting and tracing of specific DNA sequences with dTALEs in living cells.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 68 citations in OpenAlex.
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- Tracing the Chromatin: From 3C to Live-Cell Imaging.Chemical & biomedical imaging · 2024Review
- Live-cell imaging of chromatin contacts opens a new window into chromatin dynamics.Epigenetics & chromatin · 2023Review
- Imaging-Based In Situ Analysis of 5-Methylcytosine at Low Repetitive Single Gene Loci with Transcription-Activator-Like Effector Probes.ACS chemical biology · 2023Article
- Visualizing the Nucleome Using the CRISPR-Cas9 System: From in vitro to in vivo.Biochemistry. Biokhimiia · 2023Review
- Review
- Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells.Nature communications · 2022Article
- A guide for single-particle chromatin tracking in live cell nuclei.Cell biology international · 2022Review
- Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.The Journal of cell biology · 2021Article
- Engineered TALE Repeats for Enhanced Imaging-Based Analysis of Cellular 5-Methylcytosine.Chembiochem : a European journal of chemical biology · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Designer Receptors for Nucleotide-Resolution Analysis of Genomic 5-Methylcytosine by Cellular Imaging.Angewandte Chemie (International ed. in English) · 2020Article
- Artificially decreasing cortical tension generates aneuploidy in mouse oocytes.Nature communications · 2020Article
- Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms.Genomics, proteomics & bioinformatics · 2019Review
- CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci.Nucleic acids research · 2018Article
- Spindle tubulin and MTOC asymmetries may explain meiotic drive in oocytes.Nature communications · 2018Article
- Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks.Nucleic acids research · 2018Article
- Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies.Angewandte Chemie (International ed. in English) · 2018Review
- Applications of the CRISPR/Cas system beyond gene editing.Biology methods & protocols · 2018Review
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7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs). We designed a recombinant dTALE (msTALE) with variable repeat domains to specifically bind a 19-bp target sequence of major satellite DNA. The msTALE was fused with green fluorescent protein (GFP) and stably expressed in mouse embryonic stem cells. Hybridization with a major satellite probe (3D-fluorescent in situ hybridization) and co-staining for known cellular structures confirmed in vivo binding of the GFP-msTALE to major satellite DNA present at nuclear chromocenters. Dual tracing of major satellite DNA and the replication machinery throughout S-phase showed co-localization during mid to late S-phase, directly demonstrating the late replication timing of major satellite DNA. Fluorescence bleaching experiments indicated a relatively stable but still dynamic binding, with mean residence times in the range of minutes. Fluorescently labeled dTALEs open new perspectives to target and trace DNA sequences and to monitor dynamic changes in subnuclear positioning as well as interactions with functional nuclear structures during cell cycle progression and cellular differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.