ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2014
Pharmacological and genetic identification of serotonin receptor subtypes on Drosophila larval heart and aorta.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- A mechanosensitive vascular niche forProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Examining the effect of manganese on physiological processes: Invertebrate models.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2022Article
- Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis).Scientific reports · 2021Article
- Smoking flies: testing the effect of tobacco cigarettes on heart function ofBiology open · 2021Article
- Serotonin Signals Modulate Mushroom Body Output Neurons for Sustaining Water-Reward Long-Term Memory inFrontiers in cell and developmental biology · 2021Article
- The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila.Invertebrate neuroscience : IN · 2020Article
- Impedance Measures and a Mounting Technique for Drosophila: Larval Movements, Heart Rate, Imaging, and Electrophysiology.Methods and protocols · 2020Article
- Effects of inhibiting mTOR with rapamycin on behavior, development, neuromuscular physiology and cardiac function in larvalBiology open · 2019Article
- The Effects of Chloride Flux onMethods and protocols · 2019Article
- Data-driven analysis of motor activity implicates 5-HT2A neurons in backward locomotion of larval Drosophila.Scientific reports · 2018Article
- Using optogenetics to assess neuroendocrine modulation of heart rate in Drosophila melanogaster larvae.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2017Article
- Melatonin increases the regularity of cardiac rhythmicity in the Drosophila heart in both wild-type and strains bearing pathogenic mutations.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Article
- Anatomy and behavioral function of serotonin receptors in Drosophila melanogaster larvae.PloS one · 2017Article
- Dm5-HTFrontiers in systems neuroscience · 2017Article
- Article
- Modulatory effects on Drosophila larva hearts: room temperature, acute and chronic cold stress.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Optogenetic stimulation of Drosophila heart rate at different temperatures and Ca2+ concentrations.Physiological reports · 2016Article
- Pharmacological identification of cholinergic receptor subtypes on Drosophila melanogaster larval heart.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Effects of serotonin on the heartbeat of pond snails in a hunger state.Biophysics (Nagoya-shi, Japan) · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotonin, 5-hydroxytryptamine (5-HT), plays various roles in the fruit fly, Drosophila melanogaster. Previous studies have shown that 5-HT modulates the heart rate in third instar larvae. However, the receptor subtypes that mediate 5-HT action in larval cardiac tissue had yet to be determined. In this study, various 5-HT agonists and antagonists were employed to determine which 5-HT receptor subtypes are responsible for the positive chronotropic effect by 5-HT. The pharmacological results demonstrate that a 5-HT2B agonist significantly increases the heart rate; however, 5-HT1A, 5-HT1B, and 5-HT7 agonists do not have a significant effect on the heart rate. Furthermore, 5-HT2 antagonist, ketanserin, markedly reduces the positive chronotropic effect of 5-HT in a dose-response manner. Furthermore, we employed genetic approaches to confirm the pharmacological results. For this purpose, we used RNA interference line to knock down 5-HT2ADro and also used 5-HT2ADro and 5-HT2BDro insertional mutation lines. The results show that 5-HT2ADro or 5-HT2BDro receptor mutations reduce the response of the heart to 5-HT. Given these results, we conclude that these 5-HT2 receptor subtypes are involved in the action of 5-HT on the heart rate in the larval stage.
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