ArticleBMC plant biology2013
The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Targeted allelic diversification of flowering genes via CRISPR-Cas enables the development of multiple early-flowering soybean lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Plant subtilases as key initiators of peptide ligand-receptor signaling.The Plant journal : for cell and molecular biology · 2026Review
- Regulatory layers of robustness of CLAVATA-WUSCHEL feedback system in stem cell homeostasis.Frontiers in plant science · 2026Review
- Research Progress of CLE and Its Prospects in Woody Plants.Plants (Basel, Switzerland) · 2025Review
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- Subtilases: a major prospect to the genome editing in horticultural crops.Frontiers in plant science · 2024Review
- Stem Cells: Engines of Plant Growth and Development.International journal of molecular sciences · 2023Review
- Comparative Mapping Combined With Map-Based Cloning of theFrontiers in plant science · 2018Article
- Dual CLAVATA3 Peptides in Arabidopsis Shoot Stem Cell Signaling.Journal of plant biology = Singmul Hakhoe chi · 2017Article
- Comparative Transcriptome Analysis of SE initial dedifferentiation in cotton of different SE capability.Scientific reports · 2017Article
- Identification and characterization of the Populus trichocarpa CLE family.BMC genomics · 2016Article
- A cascade of arabinosyltransferases controls shoot meristem size in tomato.Nature genetics · 2015Article
- Biologically active and antimicrobial peptides from plants.BioMed research international · 2015Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough it is known that CLAVATA3 (CLV3) acts as 12- and/or 13-amino acid (AA) secreted peptides to regulate the number of stem cells in shoot apical meristems (SAMs), how functional CLV3 peptides are generated and if any particular sequences are required for the processing remain largely unknown.
resultsWe developed a mass spectrometry (MS)-based in vitro assay to monitor the cleavage of heterologously produced CLV3 fusion protein. Through co-cultivation of the fusion protein with Arabidopsis seedlings, we identified two cleavage sites: the previously reported one before Arg70 and a new one before Met39. Using synthetic peptides together with MALDI-Tof-MS analyses, we demonstrated that the non-conserved 5-AA motifs flanking N-termini of the CLV3 and its orthologous CLE1 peptides were critical for their cleavages and optimal activities in vitro. We also found that substitutions of Leu69 by Ala in fusion protein and in synthetic peptide of CLV3 compromised their cleavages, leading to significantly reduced activities in regulating the sizes of shoot and root meristems.
conclusionsThese results suggest that 5-AA residues flanking the N-terminus of CLV3 peptide are required for proper cleavages and optimal function in stem cell regulation.
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