ArticleThe Journal of cell biology2013
In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Mechanistic insights into the basis of widespread RNA localization.Nature cell biology · 2024Review
- Linking transport and translation of mRNAs with endosomes and mitochondria.EMBO reports · 2021Review
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Matrix-screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins.Nucleic acids research · 2021Article
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- Mammalian Neuronal mRNA Transport Complexes: The Few Knowns and the Many Unknowns.Frontiers in integrative neuroscience · 2021Review
- Review
- Article
- Myosin-Driven Intracellular Transport.Cold Spring Harbor perspectives in biology · 2018Review
- Regulation of class V myosin.Cellular and molecular life sciences : CMLS · 2018Review
- Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.Nature structural & molecular biology · 2017Article
- In the right place at the right time: visualizing and understanding mRNA localization.Nature reviews. Molecular cell biology · 2015Review
- Hooking She3p onto She2p for myosin-mediated cytoplasmic mRNA transport.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.RNA biology · 2015Article
- The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.The Journal of cell biology · 2014Article
- A single molecule approach to mRNA transport by a class V myosin.RNA biology · 2014Article
- Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.RNA biology · 2014Review
- Here, there, everywhere. mRNA localization in budding yeast.RNA biology · 2014Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
The assembly and composition of ribonucleic acid (RNA)-transporting particles for asymmetric messenger RNA (mRNA) localization is not well understood. During mitosis of budding yeast, the Swi5p-dependent HO expression (SHE) complex transports a set of mRNAs into the daughter cell. We recombinantly reconstituted the core SHE complex and assessed its properties. The cytoplasmic precomplex contains only one motor and is unable to support continuous transport. However, a defined interaction with a second, RNA-bound precomplex after its nuclear export dimerizes the motor and activates processive RNA transport. The run length observed in vitro is compatible with long-distance transport in vivo. Surprisingly, SHE complexes that either contain or lack RNA cargo show similar motility properties, demonstrating that the RNA-binding protein and not its cargo activates motility. We further show that SHE complexes have a defined size but multimerize into variable particles upon binding of RNAs with multiple localization elements. Based on these findings, we provide an estimate of number, size, and composition of such multimeric SHE particles in the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.