ArticleThe Plant cell2013
Abscisic acid-responsive guard cell metabolomes of Arabidopsis wild-type and gpa1 G-protein mutants.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 84 citations in OpenAlex.
- Stomatal movement as an emergent property of the remarkable plasticity of guard cells.The New phytologist · 2026Article
- A drought stress-responsive metabolite malate modulates stomatal responses through G-protein-dependent pathway in grapevine and Arabidopsis.Molecular horticulture · 2026Article
- Salmonella enterica exploits the auxin signaling pathway to overcome stomatal immunity.PLoS pathogens · 2025Article
- Apoplastic metabolomics reveals sugars as mesophyll messengers regulating guard cell ion transport under red light.Nature plants · 2025Article
- Wheat TaPYL9-involved signalling pathway impacts plant drought response through regulating distinct osmotic stress-associated physiological indices.Plant biotechnology journal · 2025Article
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Genome-Wide Identification, Expression and Interaction Analysis of GmSnRK2 and Type A PP2C Genes in Response to Abscisic Acid Treatment and Drought Stress in Soybean Plant.International journal of molecular sciences · 2022Article
- Advances in Plant Metabolomics and Its Applications in Stress and Single-Cell Biology.International journal of molecular sciences · 2022Review
- Non-autonomous stomatal control by pavement cell turgor via the K+ channel subunit AtKC1.The Plant cell · 2022Article
- Better NOT together: single-cell transcriptomic landscape of leaf tissues.Plant physiology · 2022Article
- Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells.Plant physiology · 2022Article
- Article
- The Role of Gβ Protein in Controlling Cell Expansion via Potential Interaction with Lipid Metabolic Pathways.Plant physiology · 2019Article
- Mutation of RGG2, which encodes a type B heterotrimeric G protein γ subunit, increases grain size and yield production in rice.Plant biotechnology journal · 2019Article
- Transcriptomics of Arabidopsis sperm cells at single-cell resolution.Plant reproduction · 2019Article
- G protein subunit phosphorylation as a regulatory mechanism in heterotrimeric G protein signaling in mammals, yeast, and plants.The Biochemical journal · 2018Review
- Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics.Plant physiology · 2018Article
- Characterization of thiol-based redox modifications ofFEBS open bio · 2018Article
- The G ProteinPlant physiology · 2018Article
- Genetic and Systematic Approaches Toward G Protein-Coupled Abiotic Stress Signaling in Plants.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Individual metabolites have been implicated in abscisic acid (ABA) signaling in guard cells, but a metabolite profile of this specialized cell type is lacking. We used liquid chromatography-multiple reaction monitoring mass spectrometry for targeted analysis of 85 signaling-related metabolites in Arabidopsis thaliana guard cell protoplasts over a time course of ABA treatment. The analysis utilized ∼ 350 million guard cell protoplasts from ∼ 30,000 plants of the Arabidopsis Columbia accession (Col) wild type and the heterotrimeric G-protein α subunit mutant, gpa1, which has ABA-hyposensitive stomata. These metabolomes revealed coordinated regulation of signaling metabolites in unrelated biochemical pathways. Metabolites clustered into different temporal modules in Col versus gpa1, with fewer metabolites showing ABA-altered profiles in gpa1. Ca(2+)-mobilizing agents sphingosine-1-phosphate and cyclic adenosine diphosphate ribose exhibited weaker ABA-stimulated increases in gpa1. Hormone metabolites were responsive to ABA, with generally greater responsiveness in Col than in gpa1. Most hormones also showed different ABA responses in guard cell versus mesophyll cell metabolomes. These findings suggest that ABA functions upstream to regulate other hormones, and are also consistent with G proteins modulating multiple hormonal signaling pathways. In particular, indole-3-acetic acid levels declined after ABA treatment in Col but not gpa1 guard cells. Consistent with this observation, the auxin antagonist α-(phenyl ethyl-2-one)-indole-3-acetic acid enhanced ABA-regulated stomatal movement and restored partial ABA sensitivity to gpa1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.