ReviewHuman cell2014
How to isolate urothelial cells? Comparison of four different methods and literature review.
Review in Human cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Construction of Large-Scale Bioengineered Hair Germs and In Vivo Transplantation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Electroacupuncture improves cyclophosphamide-induced bladder overactivity by reducing mechanotransduction in the rat urothelium.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2025Article
- Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells.Frontiers in bioengineering and biotechnology · 2025Article
- Canine urothelial cell model to study intracellular bacterial community development by uropathogenic Escherichia coli.PloS one · 2025Article
- Unlocking the Potential of Collagenases: Structures, Functions, and Emerging Therapeutic Horizons.Biodesign research · 2024Article
- Single-cell profiling reveals various types of interstitial cells in the bladder.Cell proliferation · 2023Article
- Advances in the bladder cancer research using 3D culture models.Bladder (San Francisco, Calif.) · 2023Review
- Novel strategy for primary epithelial cell isolation: Combination of hyaluronidase and collagenase I.Cell proliferation · 2023Article
- Characterization of the Peri-Membrane Fluorescence Phenomenon Allowing the Detection of Urothelial Tumor Cells in Urine.Cancers · 2022Article
- Specialized pro-resolution mediators in the bladder: Receptor expression and recovery of bladder function from cystitis.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Tissue engineering in reconstructive urology-The current status and critical insights to set future directions-critical review.Frontiers in bioengineering and biotechnology · 2022Review
- Innervation: the missing link for biofabricated tissues and organs.NPJ Regenerative medicine · 2020Review
- NLRP3 Promotes Diabetic Bladder Dysfunction and Changes in Symptom-Specific Bladder Innervation.Diabetes · 2019Article
- Calcium Pyrophosphate And Monosodium Urate Activate The NLRP3 Inflammasome Within Bladder Urothelium Via Reactive Oxygen Species And TXNIP.Research and reports in urology · 2019Article
- Bladder fibrosis during outlet obstruction is triggered through the NLRP3 inflammasome and the production of IL-1β.American journal of physiology. Renal physiology · 2017Article
- Biofabrication and biomaterials for urinary tract reconstruction.Research and reports in urology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study is to present the comparison of four different methods for urothelial cell isolation and culture and compare them to methods cited in the literature. Four different techniques were examined for urothelium isolation from rat bladders. Isolation effectiveness was calculated using trypan blue assay. Confirmation of isolated cell phenotype and comparison with native bladder tissue was confirmed using immunohistochemical (IHC), immunocytochemical (ICC) and immunofluorescence (IF) analysis. The method with bladder inversion and collagenase P digestion resulted in the highest number of isolated cells. These cells showed positive expression of cytokeratin 7, 8, 18, α6-integrin and p63. Our results and the literature review showed that the best method for urothelium bladder isolation is dissection of the epithelium layer from other bladder parts and digestion of mechanically prepared tissue in a collagenase solution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.