ArticleBiochimica et biophysica acta2014
ArdA proteins from different mobile genetic elements can bind to the EcoKI Type I DNA methyltransferase of E. coli K12.
Article in Biochimica et biophysica acta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
- A bacteriophage-encoded protein gp119 suppresses deoxyinosine repair to protect hypermutated viral genome.iScience · 2026Article
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- It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.Current opinion in microbiology · 2024Review
- Inhibitors of bacterial immune systems: discovery, mechanisms and applications.Nature reviews. Genetics · 2024Review
- Anti-Restriction Gene Homologs Are Highly Represented in Methicillin-Resistant and Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Identification and Characterisation of pST1023 A Mosaic, Multidrug-Resistant and Mobilisable IncR Plasmid.Microorganisms · 2022Article
- Article
- Functional comparison of anti-restriction and anti-methylation activities of ArdA, KlcA, and KlcAFrontiers in cellular and infection microbiology · 2022Article
- Comparative Genomics Provides Insight into the Function of Broad-Host Range Sponge Symbionts.mBio · 2021Article
- Plasmid replication-associated single-strand-specific methyltransferases.Nucleic acids research · 2020Article
- Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level.Genes · 2020Review
- IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR-Cas during conjugation.Nucleic acids research · 2020Article
- Analysis of indigenous plasmid sequences ofJournal of genetics · 2020Article
- Genome-wide correlation analysis suggests different roles of CRISPR-Cas systems in the acquisition of antibiotic resistance genes in diverse species.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Staphylococcal Plasmids, Transposable and Integrative Elements.Microbiology spectrum · 2018Review
- High genomic variability in the plant pathogenic bacterium Pectobacterium parmentieri deciphered from de novo assembled complete genomes.BMC genomics · 2018Article
- Comparison between complete genomes of an isolate of Pseudomonas syringae pv. actinidiae from Japan and a New Zealand isolate of the pandemic lineage.Scientific reports · 2018Article
- Polyvalent Proteins, a Pervasive Theme in the Intergenomic Biological Conflicts of Bacteriophages and Conjugative Elements.Journal of bacteriology · 2017Article
- Anti-Restriction Protein, KlcAFrontiers in cellular and infection microbiology · 2017Article
- Staphylococci on ICE: Overlooked agents of horizontal gene transfer.Mobile genetic elements · 2017Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Anti-restriction and anti-modification (anti-RM) is the ability to prevent cleavage by DNA restriction-modification (RM) systems of foreign DNA entering a new bacterial host. The evolutionary consequence of anti-RM is the enhanced dissemination of mobile genetic elements. Homologues of ArdA anti-RM proteins are encoded by genes present in many mobile genetic elements such as conjugative plasmids and transposons within bacterial genomes. The ArdA proteins cause anti-RM by mimicking the DNA structure bound by Type I RM enzymes. We have investigated ArdA proteins from the genomes of Enterococcus faecalis V583, Staphylococcus aureus Mu50 and Bacteroides fragilis NCTC 9343, and compared them to the ArdA protein expressed by the conjugative transposon Tn916. We find that despite having very different structural stability and secondary structure content, they can all bind to the EcoKI methyltransferase, a core component of the EcoKI Type I RM system. This finding indicates that the less structured ArdA proteins become fully folded upon binding. The ability of ArdA from diverse mobile elements to inhibit Type I RM systems from other bacteria suggests that they are an advantage for transfer not only between closely-related bacteria but also between more distantly related bacterial species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.