ArticleTissue engineering. Part A2014
Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Anti-miRNA Oligonucleotide Therapy for Chondrosarcoma.Molecular cancer therapeutics · 2019Article
- Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures.International journal of nanomedicine · 2019Article
- Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.Molecular biotechnology · 2018Review
- Preparation of cationized gelatin nanospheres incorporating molecular beacon to visualize cell apoptosis.Scientific reports · 2018Article
- Synthesis and Characterization of a Magnetically ActiveBioconjugate chemistry · 2018Article
- A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment.Scientific reports · 2015Article
- Temporal heterogeneity in single-cell gene expression and mechanical properties during adipogenic differentiation.Journal of biomechanics · 2015Article
- Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential.Stem cell research & therapy · 2014Article
- Retraction of Desai, et al. Tissue Engineering, Part A, 19;1/2:40-48.Tissue engineering. Part A · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Adipose-derived stem cells (ASCs) are a widely investigated type of mesenchymal stem cells with great potential for musculoskeletal regeneration. However, the use of ASCs is complicated by their cellular heterogeneity, which exists at both the population and single-cell levels. This study demonstrates a live-cell assay to investigate gene expression in ASCs undergoing osteogenesis using fluorescently tagged DNA hybridization probes called molecular beacons. A molecular beacon was designed to target the mRNA sequence for alkaline phosphatase (ALPL), a gene characteristically expressed during early osteogenesis. The percentage of cells expressing this gene in a population was monitored daily to quantify the uniformity of the differentiation process. Differentiating ASC populations were repeatedly measured in a nondestructive fashion over a 10-day period to obtain temporal gene expression data. Results showed consistent expression patterns for the investigated osteogenic genes in response to induction medium. Peak signal level, indicating when the most cells expressed ALPL at once, was observed on days 3-5. The differentiation response of sample populations was generally uniform when assessed on a well-by-well basis over time. The expression of alkaline phosphatase is consistent with previous studies of osteogenic differentiation, suggesting that molecular beacons are a viable means of monitoring the spatiotemporal gene expression of live, differentiating ASCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.