ArticlePloS one2013
High temperature inhibits ascorbate recycling and light stimulation of the ascorbate pool in tomato despite increased expression of biosynthesis genes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The SlDREBA4-SlASIL2 module mediates tomato thermotolerance by regulating the expression of SlHSP20/90.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Effects of Different Temperatures on Flavonoid Secondary Metabolites and Antioxidant Activities ofAntioxidants (Basel, Switzerland) · 2026Article
- Signaling mechanisms governing the environmental modulation of fruit quality development.Horticulture research · 2026Article
- Postharvest senescence of green vegetables and the role of glutathione metabolism: current state of knowledge and future research opportunities.Frontiers in plant science · 2026Review
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- Modelling metabolic fluxes of tomato stems reveals that nitrogen shapes central metabolism for defence against Botrytis cinerea.Journal of experimental botany · 2024Article
- Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures.Plants (Basel, Switzerland) · 2023Article
- Duodenal Metabolic Profile Changes in Heat-Stressed Broilers.Animals : an open access journal from MDPI · 2022Article
- Scion and Rootstock Differently Influence Growth, Yield and Quality Characteristics of Cherry Tomato.Plants (Basel, Switzerland) · 2020Article
- Effects of Hot Air Treatments on Postharvest Storage of Newhall Navel Orange.Plants (Basel, Switzerland) · 2020Article
- Regulation of Ascorbate Accumulation and Metabolism in Lettuce by the Red:Blue Ratio of Continuous Light Using LEDs.Frontiers in plant science · 2020Article
- Article
- Article
- Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit.Frontiers in plant science · 2019Review
- Putting primary metabolism into perspective to obtain better fruits.Annals of botany · 2018Review
- Vitamin C Content in Fruits: Biosynthesis and Regulation.Frontiers in plant science · 2018Review
- Differentially expressed protein and gene analysis revealed the effects of temperature on changes in ascorbic acid metabolism in harvested tea leaves.Horticulture research · 2018Article
- Rice GDP-mannose pyrophosphorylase OsVTC1-1 and OsVTC1-3 play different roles in ascorbic acid synthesis.Plant molecular biology · 2016Article
- Ascorbate biosynthesis and its involvement in stress tolerance and plant development in rice (Oryza sativa L.).Plant molecular biology · 2015Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding how the fruit microclimate affects ascorbate (AsA) biosynthesis, oxidation and recycling is a great challenge in improving fruit nutritional quality. For this purpose, tomatoes at breaker stage were harvested and placed in controlled environment conditions at different temperatures (12, 17, 23, 27 and 31 °C) and irradiance regimes (darkness or 150 µmol m(-2) s(-1)). Fruit pericarp tissue was used to assay ascorbate, glutathione, enzymes related to oxidative stress and the AsA/glutathione cycle and follow the expression of genes coding for 5 enzymes of the AsA biosynthesis pathway (GME, VTC2, GPP, L-GalDH, GLDH). The AsA pool size in pericarp tissue was significantly higher under light at temperatures below 27 °C. In addition, light promoted glutathione accumulation at low and high temperatures. At 12 °C, increased AsA content was correlated with the enhanced expression of all genes of the biosynthesis pathway studied, combined with higher DHAR and MDHAR activities and increased enzymatic activities related to oxidative stress (CAT and APX). In contrast, at 31 °C, MDHAR and GR activities were significantly reduced under light indicating that enzymes of the AsA/glutathione cycle may limit AsA recycling and pool size in fruit pericarp, despite enhanced expression of genes coding for AsA biosynthesis enzymes. In conclusion, this study confirms the important role of fruit microclimate in the regulation of fruit pericarp AsA content, as under oxidative conditions (12 °C, light) total fruit pericarp AsA content increased up to 71%. Moreover, it reveals that light and temperature interact to regulate both AsA biosynthesis gene expression in tomato fruits and AsA oxidation and recycling.
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