ArticlePloS one2013
Heat shock modulates neutrophil motility in zebrafish.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Physiological Effects, Molecular Regulation, and Adaptive Strategies of Heat Stress in Fish Under Global Warming.Animals : an open access journal from MDPI · 2026Review
- Time-Resolved Proteomic Analysis in Zebrafish Using Bioorthogonal Noncanonical Amino Acid Tagging.Journal of proteome research · 2026Article
- Characterizing trajectories of innate immune cells in larval zebrafish.bioRxiv : the preprint server for biology · 2025Article
- ThemBio · 2025Article
- The innate immune regulator MyD88 dampens fibrosis during zebrafish heart regeneration.Nature cardiovascular research · 2024Article
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish.Biology · 2023Review
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
- Article
- Cell Type-Specific Transcriptome Profiling Reveals a Role for Thioredoxin During Tumor Initiation.Frontiers in immunology · 2022Article
- A mutant wfs1 zebrafish model of Wolfram syndrome manifesting visual dysfunction and developmental delay.Scientific reports · 2021Article
- Cell type specific gene expression profiling reveals a role for complement component C3 in neutrophil responses to tissue damage.Scientific reports · 2020Article
- Review
- Swimming motility of a gut bacterial symbiont promotes resistance to intestinal expulsion and enhances inflammation.PLoS biology · 2020Article
- Yeast and Filaments Have Specialized, Independent Activities in a Zebrafish Model of Candida albicans Infection.Infection and immunity · 2018Article
- Use of zebrafish to studyDisease models & mechanisms · 2018Review
- Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses.Journal of visualized experiments : JoVE · 2017Article
- Rac2 Functions in Both Neutrophils and Macrophages To Mediate Motility and Host Defense in Larval Zebrafish.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Matrix metalloproteinase 9 modulates collagen matrices and wound repair.Development (Cambridge, England) · 2015Article
- Behavioral and proteomic analysis of stress response in zebrafish (Danio rerio).Journal of proteome research · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock is a routine method used for inducible gene expression in animal models including zebrafish. Environmental temperature plays an important role in the immune system and infection progression of ectotherms. In this study, we analyzed the impact of short-term heat shock on neutrophil function using zebrafish (Danio rerio) as an animal model. Short-term heat shock decreased neutrophil recruitment to localized Streptococcus iniae infection and tail fin wounding. Heat shock also increased random neutrophil motility transiently and increased the number of circulating neutrophils. With the use of the translating ribosome affinity purification (TRAP) method for RNA isolation from specific cell types such as neutrophils, macrophages and epithelial cells, we found that heat shock induced the immediate expression of heat shock protein 70 (hsp70) and a prolonged expression of heat shock protein 27 (hsp27). Heat shock also induced cell stress as detected by the splicing of X-box binding protein 1 (xbp1) mRNA, a marker for endoplasmic reticulum (ER) stress. Exogenous expression of Hsp70, Hsp27 and spliced Xbp1 in neutrophils or epithelial cells did not reproduce the heat shock induced effects on neutrophil recruitment. The effect of heat shock on neutrophils is likely due to a combination of complex changes, including, but not limited to changes in gene expression. Our results indicate that routine heat shock can alter neutrophil function in zebrafish. The findings suggest that caution should be taken when employing a heat shock-dependent inducible system to study the innate immune response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.