Evidence map›Paper›PMID 24367590›Full record

ArticlePloS one2013

Keratinocyte growth factor gene delivery via mesenchymal stem cells protects against lipopolysaccharide-induced acute lung injury in mice.

Jie Chen, Chunsun Li, Xiaofang Gao, Chonghui Li, Zhixin Liang, Ling Yu, Yanqin Li, Xiaoyi Xiao, Liangan Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jie ChenDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Chunsun LiDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Xiaofang GaoDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Chonghui LiDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Zhixin LiangDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Ling YuDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Yanqin LiDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Xiaoyi XiaoDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Liangan ChenDepartment of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing, China.
Chinese People's Liberation Army · CNPeople's Liberation Army No. 150 Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are associated with high morbidity and mortality, and have no specific therapy. Keratinocyte growth factor (KGF) is a critical factor for pulmonary epithelial repair and acts via the stimulation of epithelial cell proliferation. Mesenchymal stem cells (MSCs) have been proved as good therapeutic vectors. Thus, we hypothesized that MSC-based KGF gene therapy would have beneficial effects on lipopolysaccharide(LPS)-induced lung injury. After two hours of intratracheal LPS administration to induce lung injury, mice received saline, MSCs alone, empty vector-engineered MSCs (MSCs-vec) or KGF-engineered MSCs (MSCs-kgf) via the tail vein. The MSCs-kgf could be detected in the recipient lungs and the level of KGF expression significantly increased in the MSCs-kgf mice. The MSC-mediated administration of KGF not only improved pulmonary microvascular permeability but also mediated a down-regulation of proinflammatory responses (reducing IL-1β and TNF-α) and an up-regulation of anti-inflammatory responses (increasing cytokine IL-10). Furthermore, the total severity scores of lung injury were significantly reduced in the MSCs-kgf group compared with the other three groups. The underlying mechanism of the protective effect of KGF on ALI may be attributed to the promotion of type II lung epithelial cell proliferation and the enhancement of surfactant synthesis. These findings suggest that MSCs-based KGF gene therapy may be a promising strategy for ALI treatment.

Indexed as

Genetic TherapyGene Transfer TechniquesMesenchymal Stem Cell TransplantationAnimalsCell ProliferationCytoprotectionEpithelial CellsFibroblast Growth Factor 7Gene Expression RegulationLipopolysaccharidesLungLung InjuryMaleMembrane ProteinsMiceProliferating Cell Nuclear AntigenFibroblast Growth Factor 7LipopolysaccharidesMembrane ProteinsProliferating Cell Nuclear AntigenRNA, Messenger

Identifiers

PMID24367590
PMCPMC3867420
OpenAlexW2046542438

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.