ArticlePloS one2013
High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 49 citations in OpenAlex.
- Signatures of local adaptation and maladaptation to future climate in wild Zizania latifolia.Communications biology · 2024Article
- Weed genomics: yielding insights into the genetics of weedy traits for crop improvement.aBIOTECH · 2023Review
- DomesticatingFrontiers in plant science · 2023Article
- Supporting in situ conservation of the genetic diversity of crop wild relatives using genomic technologies.Molecular ecology · 2022Review
- Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.International journal of molecular sciences · 2022Review
- Progress of Genomics-Driven Approaches for Sustaining Underutilized Legume Crops in the Post-Genomic Era.Frontiers in genetics · 2022Review
- Potential of Platinum Standard Reference Genomes to Exploit Natural Variation in the Wild Relatives of Rice.Frontiers in plant science · 2020Review
- Genetic diversity ofEcology and evolution · 2020Article
- DomesticatingFrontiers in plant science · 2019Article
- Australian Wild Rice Populations: A Key Resource for Global Food Security.Frontiers in plant science · 2019Review
- Neo-Domestication of an Interspecific TetraploidGenes · 2018Article
- Genomics of crop wild relatives: expanding the gene pool for crop improvement.Plant biotechnology journal · 2016Review
- Genomics strategies for germplasm characterization and the development of climate resilient crops.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice-like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction with high throughput sequencing of genes for key domestication traits. While M. stipoides has previously been identified as having potential as a new grain crop for human consumption, only a limited understanding of its genetic diversity and breeding system was available to aid the domestication process. Next generation sequencing of deeply-pooled target amplicons estimated allelic diversity of a selected base population at 14.3 SNP/Mb and identified novel, putatively mutation-induced polymorphisms at about 2.4 mutations/Mb. A 97% lethal dose (LD₉₇) of ethyl methanesulfonate treatment was applied without inducing sterility in this polyploid species. Forward and reverse genetic screens identified beneficial alleles for the domestication trait, seed-shattering. Unique phenotypes observed in the M2 population suggest the potential for rapid accumulation of beneficial traits without recourse to a traditional cross-breeding strategy. This approach may be applicable to other wild species, unlocking their potential as new food, fibre and fuel crops.
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