ArticlePLoS genetics2013
Base pairing interaction between 5'- and 3'-UTRs controls icaR mRNA translation in Staphylococcus aureus.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 102 citations in OpenAlex.
- Long-range mRNA folding shapes expression and sequence of bacterial genes.Molecular cell · 2026Article
- Deciphering the comprehensive relationship between 5' UTR and 3' UTR sequences with deep learning.Bioinformatics (Oxford, England) · 2026Article
- Biofilm Production, Distribution ofAntibiotics (Basel, Switzerland) · 2025Article
- Long-range mRNA folding shapes expression and sequence of bacterial genes.bioRxiv : the preprint server for biology · 2025Article
- A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis.Nucleic acids research · 2025Article
- PIPETS: a statistically informed, gene-annotation agnostic analysis method to study bacterial termination using 3'-end sequencing.BMC bioinformatics · 2024Article
- PIPETS: A statistically informed, gene-annotation agnostic analysis method to study bacterial termination using 3'-end sequencing.bioRxiv : the preprint server for biology · 2024Article
- Complex Regulation of Gamma-Hemolysin Expression Impacts Staphylococcus aureus Virulence.Microbiology spectrum · 2023Article
- A network of small RNAs regulates sporulation initiation in Clostridioides difficile.The EMBO journal · 2023Article
- Small RNAs Activate Salmonella Pathogenicity Island 1 by Modulating mRNA Stability through theJournal of bacteriology · 2023Article
- RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3'UTR required for intermediate vancomycin resistance.Nature communications · 2022Article
- The Transcription Factor SpoVG Is of Major Importance for Biofilm Formation ofInternational journal of molecular sciences · 2022Article
- Experimental Polymorphism Survey in Intergenic Regions of theMicroorganisms · 2022Article
- Regulation of Heterogenous LexA Expression inInternational journal of molecular sciences · 2022Article
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- Critical Role of 3'-Downstream Region ofMicroorganisms · 2021Article
- Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies.Microbial cell (Graz, Austria) · 2021Review
- Plasmid-Chromosome Crosstalk inFrontiers in cellular and infection microbiology · 2021Article
- Staphylococcal Biofilm Development: Structure, Regulation, and Treatment Strategies.Microbiology and molecular biology reviews : MMBR · 2020Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 7 institutions in 3 countries.
Funding
Abstract
The presence of regulatory sequences in the 3' untranslated region (3'-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 3'-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 3'-UTRs longer than 100-nt and thus, potential regulatory functions. We selected the long 3'-UTR of icaR, which codes for the repressor of the main exopolysaccharidic compound of the S. aureus biofilm matrix, to evaluate the role that 3'-UTRs may play in controlling mRNA expression. We showed that base pairing between the 3'-UTR and the Shine-Dalgarno (SD) region of icaR mRNA interferes with the translation initiation complex and generates a double-stranded substrate for RNase III. Deletion or substitution of the motif (UCCCCUG) within icaR 3'-UTR was sufficient to abolish this interaction and resulted in the accumulation of IcaR repressor and inhibition of biofilm development. Our findings provide a singular example of a new potential post-transcriptional regulatory mechanism to modulate bacterial gene expression through the interaction of a 3'-UTR with the 5'-UTR of the same mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.