ArticleProceedings of the National Academy of Sciences of the United States of America2014
Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Keeping it safe: control of meiotic chromosome breakage.Trends in genetics : TIG · 2025Review
- The ATPase activity of yeast chromosome axis protein Hop1 affects the frequency of meiotic crossovers.Nucleic acids research · 2025Article
- TRIP13: A promising cancer immunotherapy target.Cancer innovation · 2024Review
- Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization.Science China. Life sciences · 2024Article
- TRIP13 localizes to synapsed chromosomes and functions as a dosage-sensitive regulator of meiosis.eLife · 2024Article
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- The N-terminal modification of HORMAD2 causes its ectopic persistence on synapsed chromosomes without meiotic blockade.Reproduction (Cambridge, England) · 2024Article
- Absence of chromosome axis protein recruitment prevents meiotic recombination chromosome-wide in the budding yeastProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chromatin binding by HORMAD proteins regulates meiotic recombination initiation.The EMBO journal · 2024Article
- Meiotic prophase length modulates Tel1-dependent DNA double-strand break interference.PLoS genetics · 2024Article
- Targeting TRIP13 for overcoming anticancer drug resistance (Review).Oncology reports · 2023Article
- Exportin-mediated nucleocytoplasmic transport maintains Pch2 homeostasis during meiosis.PLoS genetics · 2023Article
- A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori.G3 (Bethesda, Md.) · 2023Article
- The conserved AAA ATPase PCH-2 distributes its regulation of meiotic prophase events through multiple meiotic HORMADs in C. elegans.PLoS genetics · 2023Article
- TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana.Nature plants · 2023Article
- ThePNAS nexus · 2023Article
- ZYP1-mediated recruitment of PCH2 to the synaptonemal complex remodels the chromosome axis leading to crossover restriction.Nucleic acids research · 2022Article
- Review
- Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation.eLife · 2021Article
- Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis.Current genetics · 2021Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
In budding yeast the pachytene checkpoint 2 (Pch2) protein regulates meiotic chromosome axis structure by maintaining the domain-like organization of the synaptonemal complex proteins homolog pairing 1 (Hop1) and molecular zipper 1 (Zip1). Pch2 has also been shown to modulate meiotic double-strand break repair outcomes to favor recombination between homologs, play an important role in the progression of meiotic recombination, and maintain ribosomal DNA stability. Pch2 homologs are present in fruit flies, worms, and mammals, however the molecular mechanism of Pch2 function is unknown. In this study we provide a unique and detailed biochemical analysis of Pch2. We find that purified Pch2 is an AAA+ (ATPases associated with diverse cellular activities) protein that oligomerizes into single hexameric rings in the presence of nucleotides. In addition, we show Pch2 binds to Hop1, a critical axial component of the synaptonemal complex that establishes interhomolog repair bias, in a nucleotide-dependent fashion. Importantly, we demonstrate that Pch2 displaces Hop1 from large DNA substrates and that both ATP binding and hydrolysis by Pch2 are required for Pch2-Hop1 transactions. Based on these and previous cell biological observations, we suggest that Pch2 impacts meiotic chromosome function by directly regulating Hop1 localization.
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