ArticleProceedings of the National Academy of Sciences of the United States of America2014
Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 223 citations in OpenAlex.
- LRS1 phosphorylated in response to blue light regulates stomatal opening.Journal of plant research · 2026Article
- The red and blue light dialog of stomatal regulation for water-efficient crops.Plant physiology · 2026Review
- Guard cell photorespiration controls stomata behavior and development.The New phytologist · 2026Article
- Nitric Oxide Enhances Drought Tolerance inPlants (Basel, Switzerland) · 2026Article
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Was the evolution of faster stomata driven by increased gas exchange rates rather than increasing water use efficiency?The New phytologist · 2026Article
- Effects of Nanomaterials on Crops.Current issues in molecular biology · 2025Review
- Guard-cell expression of abscisic acid receptors for engineering water-use-efficient plants without trade-offs in growth.The New phytologist · 2025Article
- Crop growth model-enabled genetic mapping of biomass accumulation dynamics in photoperiod-sensitive sorghum.The plant genome · 2025Article
- Discovery of Hub Genes Involved in Seed Development and Lipid Biosynthesis in Sea Buckthorn (Plants (Basel, Switzerland) · 2025Article
- Metabolic engineering of stomatal precursor cells enhances photosynthetic water-use efficiency and vegetative growth under water-deficit conditions in Arabidopsis thaliana.Plant biotechnology journal · 2025Article
- Guard cells on the adaxial and abaxial leaf surfaces use different compositions of potassium ion channels to drive light-induced stomatal opening.Nature plants · 2025Article
- Guard cell-specific glycine decarboxylase manipulation affects Arabidopsis photosynthesis, growth and stomatal behavior.The New phytologist · 2025Article
- Impact of climate-driven changes in temperature on stomatal anatomy and physiology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- PROTON ATPASE TRANSLOCATION CONTROL 1-mediated HPNAS nexus · 2025Article
- A C4 plant K+ channel accelerates stomata to enhance C3 photosynthesis and water use efficiency.Plant physiology · 2025Article
- Article
- Potential mechanisms for the rapid post-drought reversal of ABA-induced stomatal closure by melatonin, 5-aminolevulinic acid, and brassinosteroids.Photosynthetica · 2025Review
- TOT3-AHA1 module: its role in fine-tuning stomatal responses.Frontiers in plant science · 2025Review
- Stomatal opening under high temperatures is controlled by the OST1-regulated TOT3-AHA1 module.Nature plants · 2025Article
40 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomatal pores surrounded by a pair of guard cells in the plant epidermis control gas exchange between plants and the atmosphere in response to light, CO2, and the plant hormone abscisic acid. Light-induced stomatal opening is mediated by at least three key components: the blue light receptor phototropin (phot1 and phot2), plasma membrane H(+)-ATPase, and plasma membrane inward-rectifying K(+) channels. Very few attempts have been made to enhance stomatal opening with the goal of increasing photosynthesis and plant growth, even though stomatal resistance is thought to be the major limiting factor for CO2 uptake by plants. Here, we show that transgenic Arabidopsis plants overexpressing H(+)-ATPase using the strong guard cell promoter GC1 showed enhanced light-induced stomatal opening, photosynthesis, and plant growth. The transgenic plants produced larger and increased numbers of rosette leaves, with ∼42-63% greater fresh and dry weights than the wild type in the first 25 d of growth. The dry weights of total flowering stems of 45-d-old transgenic plants, including seeds, siliques, and flowers, were ∼36-41% greater than those of the wild type. In addition, stomata in the transgenic plants closed normally in response to darkness and abscisic acid. In contrast, the overexpression of phototropin or inward-rectifying K(+) channels in guard cells had no effect on these phenotypes. These results demonstrate that stomatal aperture is a limiting factor in photosynthesis and plant growth, and that manipulation of stomatal opening by overexpressing H(+)-ATPase in guard cells is useful for the promotion of plant growth.
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