ArticleProceedings of the National Academy of Sciences of the United States of America2014
PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 169 citations in OpenAlex.
- The Role of RNA Interactions with PIWI in Regulating Gametogenesis, Fertilization, and Early Development Across Species.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- A chromosome-level reference genome for the colonial marine hydrozoan Podocoryna americana.G3 (Bethesda, Md.) · 2026Article
- Gene body methylation suppresses intragenic transcription and permits epigenetic inheritance in a cnidarian.Nature ecology & evolution · 2026Article
- Deciphering the ancestral mechanisms of neural regeneration through single-cell analyses of jellyfish rhopalium repair.Science advances · 2026Article
- A chromosome-level genome assembly for the colonial marine hydrozoanbioRxiv : the preprint server for biology · 2026Article
- Reproductive phasiRNAs are the piRNAs of plants.Trends in genetics : TIG · 2026Review
- Building a resilient ovarian reserve: Early soma-oocyte interactions.Current topics in developmental biology · 2026Review
- Characterization of long noncoding RNAs, microRNAs, and piwiRNAs in reef-building corals.Environmental epigenetics · 2026Article
- Identification of a piwi1 homologue gene in the orange-spotted grouper (Biochemistry and biophysics reports · 2025Article
- Examination of Germline and Somatic Intercellular Bridges in Hydra vulgaris Reveals Insights into the Evolutionarily Conserved Mechanism of Intercellular Bridge Formation.Molecular biology and evolution · 2025Article
- A molecular, spatial and regulatory atlas of the Hydra vulgaris nervous system.Development (Cambridge, England) · 2025Article
- The dynamic genomes of Hydra and the anciently active repeat complement of animal chromosomes.Genome biology · 2025Article
- Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025Review
- Evolution of piRNA-guided defense against transposable elements.Trends in genetics : TIG · 2025Review
- A Network Approach to White Band Disease Challenged Staghorn CoralEcology and evolution · 2025Article
- Article
- PIWI proteins and piRNAs: key regulators of stem cell biology.Frontiers in cell and developmental biology · 2025Review
- Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.bioRxiv : the preprint server for biology · 2024Article
- Candidate stem cell isolation and transplantation in Hexacorallia.Cell reports · 2024Article
- PIWIL1 is recruited to centrosomes during mitosis in colorectal cancer cells and is linked to cell cycle progression.Scientific reports · 2024Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) are found in animal germlines and are essential for fertility, but their functions outside of the gonad are not well understood. The cnidarian Hydra is a simple metazoan with well-characterized stem/progenitor cells that provides a unique model for analysis of PIWI function. Here we report that Hydra has two PIWI proteins, Hydra PIWI (Hywi) and Hydra PIWI-like (Hyli), both of which are expressed in all Hydra stem/progenitor cells, but not in terminally differentiated cells. We identified ∼15 million piRNAs associated with Hywi and/or Hyli and found that they exhibit the ping-pong signature of piRNA biogenesis. Hydra PIWI proteins are strictly cytoplasmic and thus likely act as posttranscriptional regulators. To explore this function, we generated a Hydra transcriptome for piRNA mapping. piRNAs map to transposons with a 25- to 35-fold enrichment compared with the abundance of transposon transcripts. By sequencing the small RNAs specific to the interstitial, ectodermal, and endodermal lineages, we found that the targeting of transposons appears to be largely restricted to the interstitial lineage. We also identified putative nontransposon targets of the pathway unique to each lineage. Finally we demonstrate that hywi function is essential in the somatic epithelial lineages. This comprehensive analysis of the PIWI-piRNA pathway in the somatic stem/progenitor cells of a nonbilaterian animal suggests that this pathway originated with broader stem cell functionality.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.