Trial reportBlood2014
Washing older blood units before transfusion reduces plasma iron and improves outcomes in experimental canine pneumonia.
Trial report in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Pooled it
- Red blood cell transfusion results in adhesion of neutrophils in human endotoxemia and in critically ill patients with sepsis.Transfusion · 2020Trial
- Older Blood Is Associated With Increased Mortality and Adverse Events in Massively Transfused Trauma Patients: Secondary Analysis of the PROPPR Trial.Annals of emergency medicine · 2019Trial
- Randomized trial of red cell washing for the prevention of transfusion-associated organ injury in cardiac surgery.British journal of anaesthesia · 2017Trial
- Effects of storage-aged red blood cell transfusions on endothelial function in hospitalized patients.Transfusion · 2015Trial
- Autologous transfusion of stored red blood cells increases pulmonary artery pressure.American journal of respiratory and critical care medicine · 2014Trial
- Coming clean: should adults undergoing cardiac surgery be transfused point-of-care washed allogeneic red blood cells?Journal of thoracic disease · 2026Article
- Donor- and unit-specific factors influencing hemolysis in stored canine and feline packed red blood cells.Frontiers in veterinary science · 2026Article
- Large Animal Models for Simulating Physiology of Transfusion of Red Cell Concentrates-A Scoping Review of The Literature.Medicina (Kaunas, Lithuania) · 2022Article
- Age of Red Cells for Transfusion and Outcomes in Patients with ARDS.Journal of clinical medicine · 2022Article
- Effect of washing units of canine red blood cells on storage lesions.Journal of veterinary internal medicine · 2022Article
- Effect of washed versus unwashed red blood cells on transfusion-related immune responses in preterm newborns.Clinical & translational immunology · 2022Article
- Dynamics of shape recovery by stored red blood cells during washing at the single cell level.Transfusion · 2020Article
- RBC Storage Lesion Studies in Humans and Experimental Models of Shock.Applied sciences (Basel, Switzerland) · 2020Article
- Contrasting effects of stored allogeneic red blood cells and their supernatants on permeability and inflammatory responses in human pulmonary endothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Liver Iron Retention Estimated from Utilization of Oral and Intravenous Radioiron in Various Anemias and Hemochromatosis in Humans.International journal of molecular sciences · 2020Article
- Impact of different standard red blood cell storage temperatures on human and canine RBC hemolysis and chromium survival.Transfusion · 2019Article
- Traditional and emerging technologies for washing and volume reducing blood products.Journal of blood medicine · 2019Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Article
Corrections and comments
- Commented on by
Authors and funding
18 authors at 7 institutions in 1 country.
Funding
Abstract
In a randomized controlled blinded trial, 2-year-old purpose-bred beagles (n = 24), with Staphylococcus aureus pneumonia, were exchanged-transfused with either 7- or 42-day-old washed or unwashed canine universal donor blood (80 mL/kg in 4 divided doses). Washing red cells (RBC) before transfusion had a significantly different effect on canine survival, multiple organ injury, plasma iron, and cell-free hemoglobin (CFH) levels depending on the age of stored blood (all, P < .05 for interactions). Washing older units of blood improved survival rates, shock score, lung injury, cardiac performance and liver function, and reduced levels of non-transferrin bound iron and plasma labile iron. In contrast, washing fresh blood worsened all these same clinical parameters and increased CFH levels. Our data indicate that transfusion of fresh blood, which results in less hemolysis, CFH, and iron release, is less toxic than transfusion of older blood in critically ill infected subjects. However, washing older blood prevented elevations in plasma circulating iron and improved survival and multiple organ injury in animals with an established pulmonary infection. Our data suggest that fresh blood should not be washed routinely because, in a setting of established infection, washed RBC are prone to release CFH and result in worsened clinical outcomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.