ArticleLaboratory investigation; a journal of technical methods and pathology2014
Intestinal epithelial apoptosis initiates gut mucosal injury during extracorporeal membrane oxygenation in the newborn piglet.
Article in Laboratory investigation; a journal of technical methods and pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Clinical applications of oXiris membranes: Targeting inflammation and renal dysfunction in ICU patients.Journal of intensive medicine · 2026Review
- Dynamics of viability and apoptotic regulation in chicken ileal explant culture.In vitro cellular & developmental biology. Animal · 2026Article
- IFABP and TFF3 as predictors for intestinal health in the perioperative setting in children with congenital heart disease.Frontiers in cardiovascular medicine · 2025Article
- Gastrointestinal injury in cardiopulmonary bypass: current insights and future directions.Frontiers in pharmacology · 2025Review
- Insight of Intestinal Fatty Acid Binding Protein as a Potential Biomarker in the Biology of Epithelial Damage of Gastrointestinal Membrane.Current protein & peptide science · 2025Review
- Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- Neutrophils: a key component in ECMO-related acute organ injury.Frontiers in immunology · 2024Review
- The Disconnect Between Extracorporeal Circulation and the Microcirculation: A Review.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2022Review
- Aptamer-based factor IXa inhibition preserves hemostasis and prevents thrombosis in a piglet model of ECMO.Molecular therapy. Nucleic acids · 2022Article
- Extracorporeal circulation impairs microcirculation perfusion and organ function.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- A Pilot Study To Establish anmSphere · 2021Observational
- N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin.Scientific reports · 2021Article
- Evaluation of the Oxiris Membrane in Cardiogenic Shock Requiring Extracorporeal Membrane Oxygenation Support: Study Protocol for a Single Center, Single-Blind, Randomized Controlled Trial.Frontiers in cardiovascular medicine · 2021Article
- Coagulopathy Characterized by Rotational Thromboelastometry in a Porcine Pediatric ECMO Model.The journal of extra-corporeal technology · 2020Article
- Targeted inhibition of thrombin attenuates murine neonatal necrotizing enterocolitis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions.Nature communications · 2019Article
- Thirty-eight-negative kinase 1 mediates trauma-induced intestinal injury and multi-organ failure.The Journal of clinical investigation · 2018Article
- Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns.Mediators of inflammation · 2018Article
- Abdominal Problems in Children with Congenital Cardiovascular Abnormalities.Balkan medical journal · 2015Article
- Current Understanding of Transfusion-associated Necrotizing Enterocolitis: Review of Clinical and Experimental Studies and a Call for More Definitive Evidence.Newborn (Clarksville, Md.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Neonates and young infants exposed to extracorporeal circulation during extracorporeal membrane oxygenation (ECMO) and cardiopulmonary bypass are at risk of developing a systemic inflammatory response syndrome with multi-organ dysfunction. We used a piglet model of ECMO to investigate the hypothesis that epithelial apoptosis is an early event that precedes villous damage during ECMO-related bowel injury. Healthy 3-week-old piglets were subjected to ECMO for up to 8 h. Epithelial apoptosis was measured in histopathological analysis, nuclear imaging, and terminal deoxynucleotidyl transferase dUTP nick end labeling. Plasma intestinal fatty acid-binding protein (I-FABP) levels were measured by enzyme immunoassay. Intestinal mast cells were isolated by fluorescence-assisted cell sorting. Cleaved caspase-8, caspase-9, phospho-p38 MAPK, and fas ligand expression were investigated by immunohistochemistry, western blots, and reverse transcriptase-quantitative PCR. Piglet ECMO was associated with increased gut epithelial apoptosis. Extensive apoptotic changes were noted on villus tips and in scattered crypt cells after 2 h of ECMO. After 8 h, the villi were denuded and apoptotic changes were evident in a majority of crypt cells. Increased circulating I-FABP levels, a marker of gut epithelial injury, showed that epithelial injury occurred during ECMO. We detected increased cleaved caspase-8, but not cleaved caspase-9, in epithelial cells indicating that the extrinsic apoptotic pathway was active. ECMO was associated with increased fas ligand expression in intestinal mast cells, which was induced through activation of the p38 mitogen-activated protein kinase. We conclude that epithelial apoptosis is an early event that initiates gut mucosal injury in a piglet model of ECMO.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.