Evidence map›Paper›PMID 24365703›Full record

ArticleActa biomaterialia2014

Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs.

Erica J Levorson, Marco Santoro, F Kurtis Kasper, Antonios G Mikos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Principles of Indirect Co-culture Method Using Transwell.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Probing Multicellular Tissue Fusion of Cocultured Spheroids-A 3D-Bioassembly Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021
    Article
  14. Review
  15. [Tetrahedral Framework Nucleic Acids and Human Health].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2021
    Review
  16. Review
  17. Chondrogenesis of cocultures of mesenchymal stem cells and articular chondrocytes in poly(l-lysine)-loaded hydrogels.Journal of controlled release : official journal of the Controlled Release Society · 2020
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Erica J LevorsonRice University, Department of Bioengineering, MS-142, 6100 Main Street, Houston, TX 77005-1892, USA.
Marco SantoroRice University, Department of Chemical and Biomolecular Engineering, MS-362, 6100 Main Street, Houston, TX 77005-1827, USA.
F Kurtis KasperRice University, Department of Bioengineering, MS-142, 6100 Main Street, Houston, TX 77005-1892, USA.
Antonios G MikosRice University, Department of Bioengineering, MS-142, 6100 Main Street, Houston, TX 77005-1892, USA; Rice University, Department of Chemical and Biomolecular Engineering, MS-362, 6100 Main Street, Houston, TX 77005-1827, USA. Electronic address: mikos@rice.edu.

Funding

Flow Perfusion Bioreactor Fabrication of Bioactive Polymer/ECM Hybrid ConstructsR01AR057083 · NIAMS · RICE UNIVERSITY · PI MIKOS, ANTONIOS G. · 2009 to 2013
$1.6M
NIAMS NIH HHS R01 AR057083NIAMS NIH HHS R01AR57083
6 · The paper itself

Abstract

In this work, the influence of direct cell-cell contact in co-cultures of mesenchymal stem cells (MSCs) and chondrocytes for the improved deposition of cartilage-like extracellular matrix (ECM) within nonwoven fibrous poly(∊-caprolactone) (PCL) scaffolds was examined. To this end, chondrocytes and MSCs were either co-cultured in direct contact by mixing on a single PCL scaffold or produced via indirect co-culture, whereby the two cell types were seeded on separate scaffolds which were then cultured together in the same system either statically or under media perfusion in a bioreactor. In static cultures, the chondrocyte scaffold of an indirectly co-cultured group generated significantly greater amounts of glycosaminoglycan and collagen than the direct co-culture group initially seeded with the same number of chondrocytes. Furthermore, improved ECM production was linked to greater cellular proliferation and distribution throughout the scaffold in static culture. In perfusion cultures, flow had a significant effect on the proliferation of the chondrocytes. The ECM contents within the chondrocyte-containing scaffolds of the indirect co-culture groups either approximated or surpassed the amounts generated within the direct co-culture group. Additionally, within bioreactor culture there were indications that chondrocytes had an influence on the chondrogenesis of MSCs as evidenced by increases in cartilaginous ECM synthetic capacity. This work demonstrates that it is possible to generate PCL/ECM hybrid scaffolds for cartilage regeneration by utilizing the factors secreted by two different cell types, chondrocytes and MSCs, even in the absence of juxtacrine signaling.

Indexed as

AnimalsBioreactorsCattleCell CountChondrocytesCoculture TechniquesCollagenDNAExtracellular MatrixGlycosaminoglycansMesenchymal Stem CellsPolyestersRabbitsStaining and LabelingSurface PropertiesTissue ScaffoldsCollagenDNAGlycosaminoglycanspolycaprolactonePolyestersCartilage tissue engineeringChondrocyteCo-cultureExtracellular matrixMesenchymal stem cell

Identifiers

PMID24365703
PMCPMC3976699

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.