ArticleParasites & vectors2013
Molecular characterization and immune modulation properties of Clonorchis sinensis-derived RNASET2.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- The human RNASET2 alarmin-like molecule differentially affects prostate cancer cells behavior in both cell autonomous and non-cell autonomous manners.Journal of translational medicine · 2025Article
- Hypoxia Enhances the Expression of RNASET2 in Human Monocyte-Derived Dendritic Cells: Role of PI3K/AKT Pathway.International journal of molecular sciences · 2021Article
- Molecular Mechanisms ofFrontiers in cell and developmental biology · 2021Review
- In vivo and in vitro studies using Clonorchis sinensis adult-derived total protein (CsTP) on cellular function and inflammatory effect in mouse and cell model.Parasitology research · 2020Article
- Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal LeechFrontiers in immunology · 2020Article
- AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech.Journal of innate immunity · 2019Review
- Molecular characterization of triosephosphate isomerase from Echinococcus granulosus.Parasitology research · 2018Article
- Clonorchis sinensis adult-derived proteins elicit Th2 immune responses by regulating dendritic cells via mannose receptor.PLoS neglected tropical diseases · 2018Article
- Recombinant protein ofOncotarget · 2017Article
- Expression of Clonorchis sinensis GIIIsPLAParasitology research · 2017Article
- Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control.Infectious diseases of poverty · 2016Review
- Interleukin-13 is involved in the formation of liver fibrosis in Clonorchis sinensis-infected mice.Parasitology research · 2016Article
- Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro.Oncotarget · 2016Article
- Molecular identification, immunolocalization, and characterization of Clonorchis sinensis triosephosphate isomerase.Parasitology research · 2015Article
- CsRNASET2 is an important component of Clonorchis sinensis responsible for eliciting Th2 immune response.Parasitology research · 2015Article
- Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct.Parasites & vectors · 2015Article
- Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis.Parasitology research · 2014Article
- Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products.Parasitology research · 2014Article
- Molecular characterization of Clonorchis sinensis secretory myoglobin: delineating its role in anti-oxidative survival.Parasites & vectors · 2014Article
- Correlation between clonorchiasis incidences and climatic factors in Guangzhou, China.Parasites & vectors · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClonorchis sinensis (C. sinensis, Cs) is a trematode parasite that often causes chronic cumulative infections in the hepatobiliary ducts of the host and can lead to pathological changes by continuously released excretory/secretory proteins (ESPs). A T2 ribonuclease in trematode ESPs, has been identified as a potent regulator of dendritic cell (DCs) modulation. We wondered whether there was a counterpart present in CsESPs with similar activity. To gain a better understanding of CsESPs associated immune responses, we identified and characterized RNASET2 of C. sinensis (CsRNASET2) in this paper.
methodsWe expressed CsRNASET2 in Pichia pastoris and identified its molecular characteristics using bioinformatic analysis and experimental approaches. The immune modulation activities of CsRNASET2 were confirmed by evaluating cytokine production and surface markers of recombinant CsRNASET2 (rCsRNASET2) co-cultured DCs, and monitoring levels of IgG isotypes from rCsRNASET2 administered BALB/c mice.
resultsCsRNASET2 appeared to be a glycoprotein of T2 ribonuclease family harboring conserved CAS motifs and rich in B-cell epitopes. Furthermore, CsRNASET2 was present in CsESPs and was able to modulate cytokine production of DCs. In addition, rCsRNASET2 could significantly suppress the expression of lipopolysaccharide-induced DCs maturation markers. In addition, when subcutaneously administered with rCsRNASET2 there was a marked effect on IgG isotypes in mouse sera.
conclusionCollectively, we revealed that CsRNASET2, a T2 ribonuclease present in CsESPs, could modulate DCs maturation and might play an important role in C. sinensis associated immune regulation in the host.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.