ArticleStem cell research2014
Side population sorting separates subfractions of cycling and non-cycling intestinal stem cells.
Article in Stem cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Predominant Asymmetrical Stem Cell Fate Outcome Limits the Rate of Niche Succession in Human Colonic Crypts.EBioMedicine · 2018Trial
- Distinct Side Population Cell Subtypes Have Different Stemness Levels in Human Ovarian Cancer Cells.Current medical science · 2021Article
- Abcg2-expressing side population cells contribute to cardiomyocyte renewal through fusion.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Enteroendocrine Progenitor Cell-Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner.Cellular and molecular gastroenterology and hepatology · 2020Article
- Identification, isolation and characterization of human LGR5-positive colon adenoma cells.Development (Cambridge, England) · 2018Article
- Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity.Cell stem cell · 2017Article
- A label-retaining but unipotent cell population resides in biliary compartment of mammalian liver.Scientific reports · 2017Article
- Kinetic Modeling of ABCG2 Transporter Heterogeneity: A Quantitative, Single-Cell Analysis of the Side Population Assay.PLoS computational biology · 2016Article
- GI stem cells - new insights into roles in physiology and pathophysiology.The Journal of physiology · 2016Review
- Defining a stem cell hierarchy in the intestine: markers, caveats and controversies.The Journal of physiology · 2016Review
- Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments.Cell and tissue research · 2016Article
- The Role of Cardiac Side Population Cells in Cardiac Regeneration.Frontiers in cell and developmental biology · 2016Review
- Tissue underlying the intestinal epithelium elicits proliferation of intestinal stem cells following cytotoxic damage.Cell and tissue research · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We report here that side population (SP) sorting allows for the simultaneous isolation of two intestinal stem cell (ISC) subsets from wild-type (WT) mice which are phenotypically different and represent cycling and non-cycling pools of cells. Following 5-ethynyl-2'-deoxyuridine (EdU) injection, in the upper side population (USP) the percentage of EdU+ was 36% showing this fraction to be highly proliferative. In the lower side population (LSP), only 0.4% of cells were EdU+, indicating this fraction to be predominantly non-cycling. Using Lgr5-EGFP mice, we show that Lgr5-EGFP(hi) cells, representing actively cycling ISCs, are essentially exclusive to the USP. In contrast, using histone 2B-YFP mice, SP analysis revealed YFP label retaining cells (LRCs) in both the USP and the LSP. Correspondingly, evaluation of the SP fractions for mRNA markers by qRT-PCR showed that the USP was enriched in transcripts associated with both quiescent and active ISCs. In contrast, the LSP expressed mRNA markers of quiescent ISCs while being de-enriched for those of the active ISC. Both the USP and LSP are capable of generating enteroids in culture which include the four intestinal lineages. We conclude that sorting of USP and LSP fractions represents a novel isolation of cycling and non-cycling ISCs from WT mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.