ArticleJournal of extracellular vesicles2013
Toxoplasma gondii infection of fibroblasts causes the production of exosome-like vesicles containing a unique array of mRNA and miRNA transcripts compared to serum starvation.
Article in Journal of extracellular vesicles, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 78 citations in OpenAlex.
- Analysis of virulence factors in extracellular vesicles secreted by Naegleria fowleri.Parasitology research · 2024Article
- The In Vitro Promoting Angiogenesis Roles of Exosomes Derived from the Protoscoleces ofJournal of microbiology and biotechnology · 2024Article
- UnveilingFrontiers in cellular and infection microbiology · 2024Article
- Role of Extracellular Vesicles in Immunity and Host Defense.Immunological investigations · 2024Review
- Toxoplasma gondii-Derived Exosomes: A Potential Immunostimulant and Delivery System for Tumor Immunotherapy Superior to Toxoplasma gondii.International journal of nanomedicine · 2024Review
- Guidelines for the purification and characterization of extracellular vesicles of parasites.Journal of extracellular biology · 2023Article
- Extracellular Vesicles and Their Impact on the Biology of Protozoan Parasites.Tropical medicine and infectious disease · 2023Review
- Exosome-delivered miR-153 from Trichinella spiralis promotes apoptosis of intestinal epithelial cells by downregulating Bcl2.Veterinary research · 2023Article
- Infection-induced extracellular vesicles evoke neuronal transcriptional and epigenetic changes.Scientific reports · 2023Article
- The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles.Frontiers in cellular and infection microbiology · 2023Article
- Protozoa-Derived Extracellular Vesicles on Intercellular Communication with Special Emphasis onMicroorganisms · 2022Review
- Exosomal miRNA-21 from Toxoplasma gondii-infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes.Scientific reports · 2022Article
- Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells.Parasites & vectors · 2022Article
- MicroRNAs: master regulators in host-parasitic protist interactions.Open biology · 2022Review
- Exosomal RNAs: Novel Potential Biomarkers for Diseases-A Review.International journal of molecular sciences · 2022Review
- Anti-infection roles of miR-155-5p packaged in exosomes secreted by dendritic cells infected with Toxoplasma gondii.Parasites & vectors · 2022Article
- Extracellular Vesicles inJournal of immunology research · 2022Review
- Intravacuolar Pathogens Hijack Host Extracellular Vesicle Biogenesis to Secrete Virulence Factors.Frontiers in immunology · 2021Review
- Commentary: Sleep Disturbance in Bipolar Disorder: Neuroglia and Circadian Rhythms.Frontiers in psychiatry · 2021Article
- The Emerging World of Membrane Vesicles: Functional Relevance, Theranostic Avenues and Tools for Investigating Membrane Function.Frontiers in molecular biosciences · 2021Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUntil recently thought to be of little significance unless occurring during pregnancy, Toxoplasma gondii infection of human hosts is now known to play a larger role in mental health and is a growing concern in the health care community. We sought to elucidate a possible mechanism by which Toxoplasma infection may cause some of the behavioural pathology now associated with infection. We hypothesized that exosomes may be playing a role.
methodsWe utilized electron microscopy to detect the presence and size of extracellular vesicles in the supernatants of Toxoplasma-infected human foreskin fibroblasts (HFF). We then utilized microarray analysis to discern mRNA and miRNA content of the vesicles isolated from supernatants of Toxoplasma-infected (Toxo) and serum-starved (SS) HFF.
resultsWe recovered extracellular vesicles with a size consistent with exosomes that we called exosome-like vesicles (ELVs) from the supernatants of SS and Toxo cultures. The mRNA and miRNA content of these ELVs was highly regulated creating specific and unique expression profiles comparing Toxo ELVs, SS ELVs and RNA isolated from whole cell homogenates. Interestingly, among the most enriched mRNA isolated from ELVs of Toxo cells are 4 specific mRNA species that have been described in the literature as having neurologic activity: Rab-13, eukaryotic translation elongation factor 1 alpha 1, thymosin beta 4 and LLP homolog. In addition, miRNA species uniquely expressed in Toxo ELVs include miR-23b, a well-known regulator of IL-17.
conclusionWhile the production of ELVs containing mRNAs that modify behaviour are consistent with reported Toxoplasma pathology, the mechanism of enrichment and ultimate in vivo effect of these mRNA and miRNA containing ELVs remains to be investigated.
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