ArticleBlood2014
Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors.
Article in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Revolutionizing medicine: recent developments and future prospects in stem-cell therapy.International journal of surgery (London, England) · 2024Review
- Modeling of human T cell development in vitro as a read-out for hematopoietic stem cell multipotency.Biochemical Society transactions · 2021Review
- TNFSF15 facilitates human umbilical cord blood haematopoietic stem cell expansion by activating Notch signal pathway.Journal of cellular and molecular medicine · 2020Article
- Vascular Notch Signaling in Stress Hematopoiesis.Frontiers in cell and developmental biology · 2020Review
- Deficiency of Rbpj Leads to Defective Stress-Induced Hematopoietic Stem Cell Functions and Hif Mediated Activation of Non-canonical Notch Signaling Pathways.Frontiers in cell and developmental biology · 2020Article
- Understanding intrinsic hematopoietic stem cell aging.Haematologica · 2020Review
- Interleukin-23 receptor signaling mediates cancer dormancy and radioresistance in human esophageal squamous carcinoma cells via the Wnt/Notch pathway.Journal of molecular medicine (Berlin, Germany) · 2019Article
- Can genetics resolve what Notch does in HSCs?Blood · 2018Article
- Notch1 expression is regulated at the post-transcriptional level by the 3' untranslated region in hematopoietic stem cell development.International journal of hematology · 2018Article
- Endothelial jagged-2 sustains hematopoietic stem and progenitor reconstitution after myelosuppression.The Journal of clinical investigation · 2017Article
- Zebrafish In-Vivo Screening for Compounds Amplifying Hematopoietic Stem and Progenitor Cells: - Preclinical Validation in Human CD34+ Stem and Progenitor Cells.Scientific reports · 2017Article
- A small-molecule/cytokine combination enhances hematopoietic stem cell proliferation via inhibition of cell differentiation.Stem cell research & therapy · 2017Article
- Recombinant TAT-BMI-1 fusion protein induces ex vivo expansion of human umbilical cord blood-derived hematopoietic stem cells.Oncotarget · 2017Article
- Notch Signaling in the Regulation of Hematopoietic Stem Cell.Current stem cell reports · 2017Review
- The Interaction Between Niche and Hematopoietic Stem Cells.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2016Review
- From molecular interaction to acute promyelocytic leukemia: Calculating leukemogenesis and remission from endogenous molecular-cellular network.Scientific reports · 2016Article
- HoxBlinc RNA Recruits Set1/MLL Complexes to Activate Hox Gene Expression Patterns and Mesoderm Lineage Development.Cell reports · 2016Article
- Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.PloS one · 2016Article
- Article
- The NOTCH signaling pathway in normal and malignant blood cell production.Journal of cell communication and signaling · 2015Article
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7 authors.
Funding
Abstract
All blood cell lineages start from hematopoietic stem cells (HSCs), which were recently shown to represent a heterogeneous group of cells. In mice, Notch signaling promotes the maintenance of "stemness" as well as the expansion of self-renewing HSCs in vitro. Additionally, human CD34(+) cells were shown to expand in vitro in response to Notch signals. However, it is unclear whether Notch directly affects all HSCs, and whether this role is relevant in vivo. Here, we developed culture conditions that support the maintenance of CD34(+)CD133(+)CD90(low)CD38(-)CD7(-)CD10(-)CD45RA(-) (CD90(low)) cells, phenotypically defined HSCs, as well as 2 early progenitor cells (CD34(+)CD38(-)CD7(-)CD10(-)CD45RA(int) [RA(int)] and CD34(+)CD38(-)CD7(-)CD10(-)CD45RA(hi) [RA(hi)]) that were functionally equivalent to multipotent progenitor-2 and lymphoid-primed multipotent progenitor, respectively, found in cord blood. Using a genetic approach, we show that Notch signals were required for HSC preservation, with cultured HSCs being equal to ex vivo HSC cells in their ability to reconstitute immunodeficient mice; however, dnMaml-transduced HSCs were not maintained in vitro. Interestingly, Notch signaling did not appear to be required for the self-renewal of human HSCs in vivo. Our findings support the notion that Notch signals maintain human HSCs in vitro that have hematopoietic-reconstituting ability in vivo and delay the appearance of 2 newly described early progenitor cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.