ArticleJournal of bacteriology2014
The LuxR-type regulator VpsT negatively controls the transcription of rpoS, encoding the general stress response regulator, in Vibrio cholerae biofilms.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Gentamicin induction of the gonococcalmBio · 2025Article
- Parallel regulatory circuits orchestrate biofilm formation in response to c-di-GMP levels and growth phase.PLoS genetics · 2025Article
- Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins inFrontiers in microbiology · 2023Article
- Genome-wide mapping of Vibrio cholerae VpsT binding identifies a mechanism for c-di-GMP homeostasis.Nucleic acids research · 2022Article
- A putative siderophore receptor ofFrontiers in microbiology · 2022Article
- Transcriptomic Analysis Reveals That Municipal Wastewater Effluent EnhancesFrontiers in microbiology · 2021Article
- Motility of Vibrio spp.: regulation and controlling strategies.Applied microbiology and biotechnology · 2020Review
- Transcription Factor Analysis in Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Role of coaggregation in the pathogenicity and prolonged colonisation of Vibrio cholerae.Medical microbiology and immunology · 2019Article
- Transcriptional regulation of a gonococcal gene encoding a virulence factor (L-lactate permease).PLoS pathogens · 2019Article
- Cyclic di-GMP Increases Catalase Production and Hydrogen Peroxide Tolerance inApplied and environmental microbiology · 2019Article
- Exploring the Links between Nucleotide Signaling and Quorum Sensing Pathways in Regulating Bacterial Virulence.ACS infectious diseases · 2018Review
- Cyclic di-GMP Positively Regulates DNA Repair in Vibrio cholerae.Journal of bacteriology · 2018Article
- Molecular basis for the differential expression of the global regulator VieA in Vibrio cholerae biotypes directed by H-NS, LeuO and quorum sensing.Molecular microbiology · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- H-NS: an overarching regulator of the Vibrio cholerae life cycle.Research in microbiology · 2017Review
- Vibrio cholerae Biofilms and Cholera Pathogenesis.PLoS neglected tropical diseases · 2016Review
- Role of Non-coding Regulatory RNA in the Virulence of Human PathogenicFrontiers in microbiology · 2016Review
- Identification and Characterization of anFrontiers in microbiology · 2016Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Cholera is a waterborne diarrheal disease caused by Vibrio cholerae strains of serogroups O1 and O139. Expression of the general stress response regulator RpoS and formation of biofilm communities enhance the capacity of V. cholerae to persist in aquatic environments. The transition of V. cholerae between free-swimming (planktonic) and biofilm life-styles is regulated by the second messenger cyclic di-GMP (c-di-GMP). We previously reported that increasing the c-di-GMP pool by overexpression of a diguanylate cyclase diminished RpoS expression. Here we show that c-di-GMP repression of RpoS expression is eliminated by deletion of the genes vpsR and vpsT, encoding positive regulators of biofilm development. To determine the mechanism of this regulation, we constructed a strain expressing a vpsT-FLAG allele from native transcription and translation signals. Increasing the c-di-GMP pool induced vpsT-FLAG expression. The interaction between VpsT-FLAG and the rpoS promoter was demonstrated by chromatin immunoprecipitation. Furthermore, purified VpsT interacted with the rpoS promoter in a c-di-GMP-dependent manner. Primer extension analysis identified two rpoS transcription initiation sites located 43 bp (P1) and 63 bp (P2) upstream of the rpoS start codon. DNase I footprinting showed that the VpsT binding site at the rpoS promoter overlaps the primary P1 transcriptional start site. Deletion of vpsT significantly enhanced rpoS expression in V. cholerae biofilms that do not make HapR. This result suggests that VpsT and c-di-GMP contribute to the transcriptional silencing of rpoS in biofilms prior to cells entering the quorum-sensing mode.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.