Evidence map›Paper›PMID 24362364›Full record

ArticleBone marrow transplantation2014

Peripheral blood regulatory T cells in patients with diffuse systemic sclerosis (SSc) before and after autologous hematopoietic SCT: a pilot study.

J Baraut, E I Grigore, F Jean-Louis, S H Khelifa, C Durand, F Verrecchia, D Farge, L Michel

Abstract read
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In one paragraph

Article in Bone marrow transplantation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 65 citations in OpenAlex.

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  13. DNA methylation as a transcriptional regulator of the immune system.Translational research : the journal of laboratory and clinical medicine · 2019
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  14. Article
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  17. Regulatory T Cells in Systemic Sclerosis.Frontiers in immunology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

J BarautINSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France.
E I GrigoreINSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France.
F Jean-LouisINSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France.
S H KhelifaService de Médecine Interne, Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Paris, France.
C DurandService de Médecine Interne, Hôpital Hôtel Dieu, Nantes, France.
F VerrecchiaINSERM U957 and Université Nantes Atlantique, Nantes, France.
D Farge1] INSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France [2] Service de Médecine Interne, Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Paris, France.
L MichelINSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France.
Assistance Publique – Hôpitaux de Paris · FRDélégation Paris 7 · FRIMT Atlantique · FRSorbonne Paris Cité · FRUniversité Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present pilot study aims to evaluate the frequency and the function of regulatory T (Treg) cells in patients with diffuse cutaneous SSc (dcSSc) before and after autologous hematopoietic SCT (aHSCT). Peripheral blood lymphocytes from seven dcSSc patients were analyzed before and 24 months after aHSCT and were compared with those from seven healthy donors (controls). Immunophenotyping of CD4(+)CD25(high)FoxP3(+) natural Treg (nTreg), CD4(+)CD25(+)TGF-β(+) and CD4(+)CD25(+)IL-10(+) adaptive Treg (aTreg) cell subsets was performed using four-color flow cytometry. Treg-suppressive capability was measured after coculture with autologous T effector cells by evaluation of T-cell proliferation using (3)H-thymidine incorporation. Peripheral CD4(+)CD25(high)FoxP3(+) (2±0.5 vs 4.2±1.1, P<0.01), CD4(+)CD25(+)TGF-β(+) (6.9±1.8 vs 14.6±5.0, P<0.05) and CD4(+)CD25(+)IL-10(+) (10.7±0.5 vs 16.1±3.2, P<0.01) Tregs as well as CD4(+)CD25(high)CD127(low) Tregs suppressive capacity (P<0.05) were decreased in dcSSc patients vs controls. After aHSCT (n=7), the percentages of CD4(+)CD25(high)FoxP3(+) (4.1±1.8) and CD4(+)CD25(+)IL-10(+) (15.7±2.2) Treg cells and the suppressive activity of CD4(+)CD25(high)CD127(low) were restored to the levels in controls. The decreased frequency and the functional defect of peripheral Treg cells from patients with dcSSc are reversed following aHSCT to reach those observed in controls. This pilot study brings evidence of an effective restoration of nTreg and aTreg subsets, and recovery of nTreg suppressive function following aHSCT.

Indexed as

Hematopoietic Stem Cell TransplantationAdultCD4-Positive T-LymphocytesCell ProliferationCoculture TechniquesFemaleForkhead Transcription FactorsHumansImmunophenotypingInterleukin-10Interleukin-2 Receptor alpha SubunitInterleukin-7 Receptor alpha SubunitLymphocytesMaleMiddle AgedPilot ProjectsForkhead Transcription FactorsFOXP3 protein, humanInterleukin-10Interleukin-2 Receptor alpha SubunitInterleukin-7 Receptor alpha SubunitTransforming Growth Factor beta

Identifiers

PMID24362364
OpenAlexW2041179006

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.