ReviewClinical biochemistry2014
A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories.
Review in Clinical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed.
- Evolution and Evaluation of Ultra-Low Temperature Freezers: A Comprehensive Literature Review.Foods (Basel, Switzerland) · 2025Review
- Optimization of bile preparation for liquid biopsy in cholangiocarcinoma focusing on circulating tumor DNA and protein stability.Scientific reports · 2025Article
- Strategies for discovering novel hepatocellular carcinoma biomarkers.World journal of hepatology · 2025Review
- Establishing a Structured Hypospadias Biobank Cohort for Integrated Research: Methodology, Comprehensive Database Integration, and Phenotyping.Diagnostics (Basel, Switzerland) · 2025Article
- High-Throughput Single-Nucleus RNA Profiling of Minimal Puncture FFPE Samples Reveals Spatiotemporal Heterogeneity of Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The University of North Carolina Cancer Survivorship Cohort: A Resource for Collaborative Survivorship Research.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2024Article
- Deep silicification-assisted long-term preservation of structural and genomic information across biospecies: From micro to macro.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Recommendations for Method Development and Validation of qPCR and dPCR Assays in Support of Cell and Gene Therapy Drug Development.The AAPS journal · 2024Article
- Canine urothelial carcinoma: a pilot study of microRNA detection in formalin-fixed, paraffin-embedded tissue samples and in normal urine.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Fluid preservation in brain banking: a review.Free neuropathology · 2024Article
- Article
- Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts.Frontiers in cell and developmental biology · 2023Article
- Best Practices in qPCR and dPCR Validation in Regulated Bioanalytical Laboratories.The AAPS journal · 2022Article
- Long term storage of miRNA at room and elevated temperatures in a silica sol-gel matrix.RSC advances · 2021Article
- Long term conservation of DNA at ambient temperature. Implications for DNA data storage.PloS one · 2021Article
- Superiority of Formalin-Fixed Paraffin-Embedded Brain Tissue forFrontiers in neurology · 2021Article
- Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION.Viruses · 2020Article
- SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood.Lab on a chip · 2020Article
- Platform independent protein-based cell-of-origin subtyping of diffuse large B-cell lymphoma in formalin-fixed paraffin-embedded tissue.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
unlabelledFrozen biospecimens are crucial for translational research and contain well-preserved nucleic acids and protein. However, the risks of freezer failure as well as space, cost, and environmental concerns of frozen biospecimens are substantial.
objectiveThe purpose of the study was to review the current status of room temperature biospecimen storage.
methodsWe searched Pubmed and vendor websites to identify relevant information.
resultsFormalin-fixed paraffin embedded (FFPE) tissues have great value but their use is limited by cross-linking and fragmentation of nucleic acids, as well as loss of enzymatic activity. Stabilization solutions can now robustly preserve fresh tissue for up to 7days at room temperature. For longer term storage, commercial vendors of chemical matrices claim real time stability of nucleic acids of over 2 years and their accelerated aging studies to date suggest stability for 12years for RNA and 60years for DNA. However, anatomic pathology biorepositories store mostly frozen tissue rather than nucleic acids. Small quantities of tissue can be directly placed on some chemical matrices to stabilize DNA, however RNA and proteins are not preserved. Current lyophilization approaches can preserve histomorphology, DNA, RNA, and proteins though RNA shows moderate degradation after 1-2years. Formalin-free fixatives show improved but varying abilities to preserve nucleic acids and face validation as well as cost barriers in replacing FFPE specimens. The paraffin embedding process can degrade RNA.
conclusionDevelopment of robust long-term room temperature biospecimen tissue storage technology can potentially reduce costs for the biomedical community in the face of growing targeted therapy needs and decreasing budgets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.