Evidence map›Paper›PMID 24361742›Full record

ArticleOsteoarthritis and cartilage2014

A gene expression study of normal and damaged cartilage in anteromedial gonarthrosis, a phenotype of osteoarthritis.

S Snelling, R Rout, R Davidson, I Clark, A Carr, P A Hulley, A J Price

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 91 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

S SnellingThe Botnar Research Centre, University of Oxford, UK. Electronic address: sarah.snelling@ndorms.ox.ac.uk.
R RoutThe Botnar Research Centre, University of Oxford, UK.
R DavidsonBiomedical Research Unit, University of East Anglia, UK.
I ClarkBiomedical Research Unit, University of East Anglia, UK.
A CarrThe Botnar Research Centre, University of Oxford, UK.
P A HulleyThe Botnar Research Centre, University of Oxford, UK.
A J PriceThe Botnar Research Centre, University of Oxford, UK.
University of Oxford · GBUniversity of East Anglia · GB

Funding

Arthritis Research UKVersus Arthritis 19222Versus Arthritis 20087
6 · The paper itself

Abstract

objectiveTo identify osteoarthritis (OA) relevant genes and pathways in damaged and undamaged cartilage isolated from the knees of patients with anteromedial gonarthrosis (AMG) - a specific form of knee OA.

designCartilage was obtained from nine patients undergoing unicompartmental knee replacement (UKR) for AMG. AMG provides a spatial representation of OA progression; showing a reproducible and histologically validated pattern of cartilage destruction such that damaged and undamaged cartilage from within the same knee can be consistently isolated and examined. Gene expression was analysed by microarray and validated using real-time PCR.

resultsDamaged and undamaged cartilage showed distinct gene expression profiles. 754 genes showed significant up- or down-regulation (non-False discovery rate (FDR) P < 0.05) with enrichment for genes involved in cell signalling, Extracellular Matrix (ECM) and inflammatory response. A number of genes previously unreported in OA showed strongly altered expression including RARRES3, ADAMTSL2 and DUSP10. Confirmation of genes previously identified as modulated in OA was also obtained e.g., SFRP3, MMP3 and IGF1.

conclusionsThis is the first study to examine a common and consistent phenotype of OA to allow direct comparison of damaged and undamaged cartilage from within the same joint compartment. We have identified specific gene expression profiles in damaged and undamaged cartilage and have determined relevant genes and pathways in OA progression. Importantly this work also highlights the necessity for phenotypic and microanatomical characterization of cartilage in future studies of OA pathogenesis and therapeutic development.

Indexed as

AgedArthroplasty, Replacement, KneeCartilage, ArticularDisease ProgressionFemaleGene Expression ProfilingGene Expression RegulationGenetic Predisposition to DiseaseHumansMaleMiddle AgedOligonucleotide Array Sequence AnalysisOsteoarthritis, KneePhenotypeReal-Time Polymerase Chain ReactionTranscriptomeCartilageGene expressionKneeMicroarraySignalling

Identifiers

PMID24361742
PMCPMC3988961
OpenAlexW1967601085

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.