ArticleVeterinary research2013
Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations.
Article in Veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- ISAntibiotics (Basel, Switzerland) · 2025Article
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- Development of a suite ofApplied and environmental microbiology · 2024Article
- Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation.Frontiers in microbiology · 2023Review
- Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System.Applied and environmental microbiology · 2022Article
- Biostatistical prediction of genes essential for growth of Anaplasma phagocytophilum in a human promyelocytic cell line using a random transposon mutant library.Pathogens and disease · 2021Article
- Rationally designedAnimal diseases · 2021Article
- Construction ofFrontiers in microbiology · 2021Article
- Attenuation ofFrontiers in veterinary science · 2021Article
- Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro.Veterinary research · 2019Article
- Metabolic modeling of energy balances in Mycoplasma hyopneumoniae shows that pyruvate addition increases growth rate.Biotechnology and bioengineering · 2017Article
- Selective medium for culture of Mycoplasma hyopneumoniae.Veterinary microbiology · 2016Article
- Complete Genome Sequence of Mycoplasma flocculare Strain Ms42T (ATCC 27399T).Genome announcements · 2015Article
- Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid.International journal of medical microbiology : IJMM · 2014Article
- First identification of proteins involved in motility of Mycoplasma gallisepticum.Veterinary research · 2014Article
- Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Mycoplasma hyopneumoniae is the cause of enzootic pneumonia in pigs, a chronic respiratory disease associated with significant economic losses to swine producers worldwide. The molecular pathogenesis of infection is poorly understood due to the lack of genetic tools to allow manipulation of the organism and more generally for the Mycoplasma genus. The objective of this study was to develop a system for generating random transposon insertion mutants in M. hyopneumoniae that could prove a powerful tool in enabling the pathogenesis of infection to be unraveled. A novel delivery vector was constructed containing a hyperactive C9 mutant of the Himar1 transposase along with a mini transposon containing the tetracycline resistance cassette, tetM. M. hyopneumoniae strain 232 was electroporated with the construct and tetM-expressing transformants selected on agar containing tetracycline. Individual transformants contained single transposon insertions that were stable upon serial passages in broth medium. The insertion sites of 44 individual transformants were determined and confirmed disruption of several M. hyopneumoniae genes. A large pool of over 10 000 mutants was generated that should allow saturation of the M. hyopneumoniae strain 232 genome. This is the first time that transposon mutagenesis has been demonstrated in this important pathogen and could be generally applied for other Mycoplasma species that are intractable to genetic manipulation. The ability to generate random mutant libraries is a powerful tool in the further study of the pathogenesis of this important swine pathogen.
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Registered trials
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