ArticleBMC cell biology2013
Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells.
Article in BMC cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Differential functional role of Orai1 variants in constitutive CaThe Journal of biological chemistry · 2024Article
- ORAI1-Regulated Gene Expression in Breast Cancer Cells: Roles for STIM1 Binding, Calcium Influx and Transcription Factor Translocation.International journal of molecular sciences · 2022Article
- Quantification of FAM20A in human milk and identification of calcium metabolism proteins.Physiological reports · 2021Article
- NCS-1 expression is higher in basal breast cancers and regulates calcium influx and cytotoxic responses to doxorubicin.Molecular oncology · 2020Article
- Serotonin regulates maternal calcium homeostasis during the perinatal period of sheep.Journal of animal science · 2019Article
- Forms and functions of store-operated calcium entry mediators, STIM and Orai.Advances in biological regulation · 2018Review
- Constitutive calcium entry and cancer: updated views and insights.European biophysics journal : EBJ · 2017Review
- The calcium pump plasma membrane Ca(2+)-ATPase 2 (PMCA2) regulates breast cancer cell proliferation and sensitivity to doxorubicin.Scientific reports · 2016Article
- Calcium-Sensing Receptor in Breast Physiology and Cancer.Frontiers in physiology · 2016Review
- Essential role of Orai1 store-operated calcium channels in lactation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of CaExperimental and therapeutic medicine · 2015Article
- Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice.PloS one · 2014Article
- Caffeine-induced Ca(2+) oscillations in type I horizontal cell of carp retina: a mathematical model.Channels (Austin, Tex.) · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe entry of calcium ions into mammary gland epithelial cells is one of the least well-understood processes in the transport of calcium into milk during lactation. The store-operated calcium entry channel ORAI1, has been suggested as a potential mechanism for the entry of Ca(2+) into mammary gland epithelial cells from the maternal blood supply during lactation. The down regulation of the canonical ORAI1 activator STIM1 during lactation suggests that other known ORAI activators such as STIM2 and SPCA2 may be important during lactation.
resultsDifferentiation of HC11 mammary gland epithelial cells was associated with enhanced basal Ca(2+) influx. Silencing of Orai1 abolished this enhancement of Ca(2+) influx. Stim2 had a modest effect on Ca(2+) influx in this in vitro model of lactation, whereas Stim1 and Spca2 silencing had no effect. Despite pronounced increases in Spca2 mRNA during lactation there was no change in the generation of the alternative splice product generated by Mist1, which increases during lactation.
conclusionsThese studies support the hypothesis that lactation is associated with a remodelling of Ca(2+) influx and this is associated with enhancement of basal Ca(2+) influx. This enhanced Ca(2+) influx appears to occur through the calcium channel Orai1.
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