ArticlePloS one2013
A universal method for species identification of mammals utilizing next generation sequencing for the analysis of DNA mixtures.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Analysis of the microbiota of raw commercial feline diets to prioritize food safety investigations.Communications biology · 2025Article
- TICKHUNTER: A Targeted Hybridization-Capture Sequencing Approach for the Detection and Characterization of Tick-borne Pathogens and Blood Meals.bioRxiv : the preprint server for biology · 2025Article
- Amur Tiger Individual Identification Based on the Improved InceptionResNetV2.Animals : an open access journal from MDPI · 2024Article
- Review
- The development of non-destructive sampling methods of parchment skins for genetic species identification.PloS one · 2024Article
- The Food Niche Overlap and Interspecific Relationship between the Sympatric Tibetan Macaque and Grey Snub-Nosed Monkey.Animals : an open access journal from MDPI · 2023Article
- DNA-based detection of pork content in food.Heliyon · 2023Review
- Review
- A 16S Next Generation Sequencing Based Molecular and Bioinformatics Pipeline to Identify Processed Meat Products Contamination and Mislabelling.Animals : an open access journal from MDPI · 2022Article
- Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward.BioMed research international · 2022Review
- Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay.International journal of legal medicine · 2022Article
- Microbial Communities of Meat and Meat Products: An Exploratory Analysis of the Product Quality and Safety at Selected Enterprises in South Africa.Microorganisms · 2021Article
- Current issues for mammalian species identification in forensic science: a review.International journal of legal medicine · 2021Review
- Molecular identification and geographic origin of a post-Medieval elephant finding from southwestern Portugal using high-throughput sequencing.Scientific reports · 2020Article
- Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source.Foods (Basel, Switzerland) · 2020Article
- Simultaneous identification of animal-derived components in meats using high-throughput sequencing in combination with a custom-built mitochondrial genome database.Scientific reports · 2020Article
- A big data approach to metagenomics for all-food-sequencing.BMC bioinformatics · 2020Article
- Review
- A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification.Journal of analytical methods in chemistry · 2018Article
- Method for the Identification of Taxon-SpecificFrontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species identification can be interesting in a wide range of areas, for example, in forensic applications, food monitoring and in archeology. The vast majority of existing DNA typing methods developed for species determination, mainly focuses on a single species source. There are, however, many instances where all species from mixed sources need to be determined, even when the species in minority constitutes less than 1 % of the sample. The introduction of next generation sequencing opens new possibilities for such challenging samples. In this study we present a universal deep sequencing method using 454 GS Junior sequencing of a target on the mitochondrial gene 16S rRNA. The method was designed through phylogenetic analyses of DNA reference sequences from more than 300 mammal species. Experiments were performed on artificial species-species mixture samples in order to verify the method's robustness and its ability to detect all species within a mixture. The method was also tested on samples from authentic forensic casework. The results showed to be promising, discriminating over 99.9 % of mammal species and the ability to detect multiple donors within a mixture and also to detect minor components as low as 1 % of a mixed sample.
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Registered trials
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