ArticlePloS one2013
Probiotic supplementation promotes calcification in Danio rerio larvae: a molecular study.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Role of the gut microbiota in the pathogenesis and therapeutic approach to osteoporosis (Review).Biomedical reports · 2026Review
- Mitigation of PFOA-Induced Developmental Toxicity inInternational journal of molecular sciences · 2025Article
- Activation of Nrf2 by sulfuretin stimulates chondrocyte differentiation and increases bone lengths in zebrafish.BMB reports · 2023Article
- The Potential Value of Probiotics after Dental Implant Placement.Microorganisms · 2023Review
- Sustainable Ornamental Fish Aquaculture: The Implication of Microbial Feed Additives.Animals : an open access journal from MDPI · 2023Review
- First In Vivo Insights on the Effects of Tempol-Methoxycinnamate, a New UV Filter, as Alternative to Octyl Methoxycinnamate, on Zebrafish Early Development.International journal of molecular sciences · 2023Article
- Euryhaline fish larvae ingest more microplastic particles in seawater than in freshwater.Scientific reports · 2023Article
- Spirulina-enriched Substrate to Rear Black Soldier Fly (Animals : an open access journal from MDPI · 2023Article
- A zebrafish HCT116 xenograft model to predict anandamide outcomes on colorectal cancer.Cell death & disease · 2022Article
- Application of zebrafish in the study of the gut microbiome.Animal models and experimental medicine · 2022Review
- Soybean Meal-Dependent Acute Intestinal Inflammation Delays Osteogenesis in Zebrafish Larvae.International journal of molecular sciences · 2022Article
- Zebrafish caudal fin as a model to investigate the role of probiotics in bone regeneration.Scientific reports · 2022Article
- Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health.International journal of molecular sciences · 2022Article
- Appetite Regulation, Growth Performances and Fish Quality Are Modulated by Alternative Dietary Protein Ingredients in Gilthead Sea Bream (Animals : an open access journal from MDPI · 2021Article
- Dietary Synbiotics Can Help Relieve the Impacts of Deltamethrin Toxicity of Nile Tilapia Reared at Low Temperatures.Animals : an open access journal from MDPI · 2021Article
- Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Animals : an open access journal from MDPI · 2021Article
- Article
- Zebrafish Models of Human Skeletal Disorders: Embryo and Adult Swimming Together.BioMed research international · 2019Review
- Effects of Lactogen 13, a New Probiotic Preparation, on Gut Microbiota and Endocrine Signals Controlling Growth and Appetite of Oreochromis niloticus Juveniles.Microbial ecology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. We previously demonstrated that dietary supplementation with Lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in Danio rerio (zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of key genes involved in ossification, e.g. runt-related transcription factor 2 (runx2), Sp7 transcription factor (sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate (gla) protein (bglap) as well as through inhibition of sclerostin (sost), a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3-(Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation, documented an increase in Mapk1 16 days post fertilization (dpf) and of Mapk3 23 dpf in individuals receiving L. rhamnosus supplementation. Interestingly, a reduction of sost detected in the same individuals suggests that the probiotic may help treat bone disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.