ArticlePloS one2013
Reference genes selection for quantitative real-time PCR using RankAggreg method in different tissues of Capra hircus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 40 citations in OpenAlex.
- Evaluation of Reference Gene Stability in Goat Skeletal Muscle Satellite Cells during Proliferation and Differentiation Phases.Animals : an open access journal from MDPI · 2024Article
- Temporal Transcriptome Dynamics ofAnimals : an open access journal from MDPI · 2024Article
- Anti-inflammation-based treatment of atherosclerosis using Gliclazide-loaded biomimetic nanoghosts.Scientific reports · 2023Article
- QuilA® adjuvanted Coxevac® sustains Th1-CD8NPJ vaccines · 2023Article
- Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.BMC zoology · 2022Article
- Screening and validation of reference genes for qRT-PCR of bovine skeletal muscle-derived satellite cells.Scientific reports · 2022Article
- Transcriptome-based selection and validation of optimal reference genes in perirenal adipose developing of goat (Frontiers in veterinary science · 2022Article
- Genome-Wide Identification of Reference Genes for Reverse-Transcription Quantitative PCR in Goat Rumen.Animals : an open access journal from MDPI · 2021Article
- Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency.Genes & nutrition · 2021Article
- Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.Molecular biology reports · 2021Article
- Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach.BMC genomics · 2020Article
- Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats.Animals : an open access journal from MDPI · 2020Article
- Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.PloS one · 2020Article
- Selection of suitable reference genes for gene expression studies in myxosporean (Myxozoa, Cnidaria) parasites.Scientific reports · 2019Article
- Selection and validation of reference genes for quantitative Real-Time PCR in Arabis alpina.PloS one · 2019Article
- Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection.Scientific reports · 2018Article
- Fibroblast growth factor 21 induces lipolysis more efficiently than it suppresses lipogenesis in goat adipocytes.Cytotechnology · 2018Article
- Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process.Scientific reports · 2018Article
- Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium.Scientific reports · 2017Article
- Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken.PloS one · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Identification of reference genes with stable levels of gene expression is an important prerequisite for obtaining reliable results in analysis of gene expression data using quantitative real time PCR (RT-qPCR). Since the underlying assumption of reference genes is that expressed at the exact same level in all sample types, in this study, we evaluated the expression stability of nine most commonly used endogenous controls (GAPDH, ACTB, 18S rRNA, RPS18, HSP-90, ALAS, HMBS, ACAC, and B2M) in four different tissues of the domestic goat, Capra hircus, including liver, visceral, subcutaneous fat and longissimus muscles, across different experimental treatments (a standard diet prepared using the NRC computer software as control and the same diet plus one mg chromium/day). We used six different software programs for ranking of reference genes and found that individual rankings of the genes differed among them. Additionally, there was a significant difference in ranking patterns of the studied genes among different tissues. A rank aggregation method was applied to combine the ranking lists of the six programs to a consensus ranking. Our results revealed that HSP-90 was nearly always among the two most stable genes in all studied tissues. Therefore, it is recommended for accurate normalization of RT-qPCR data in goats, while GAPDH, ACTB, and RPS18 showed the most varied expressions and should be avoided as reference genes.
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