ArticleJournal of the American Society of Nephrology : JASN2014
Estimating podocyte number and density using a single histologic section.
Article in Journal of the American Society of Nephrology : JASN, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 140 citations in OpenAlex.
- Metabolic surgery mitigates early kidney injury in obese youth with diabetes by suppressing mTORC1/JAK/STAT signaling.The Journal of clinical investigation · 2026Trial
- Fetal growth restriction leads to prolonged nephrogenesis and gene dysregulation in the adult kidney.Life science alliance · 2026Article
- Impact of Apolipoprotein L1-Associated Pregnancy Complications on Kidney Disease Phenotypes in a HIV-Associated Nephropathy Mouse Model.Kidney360 · 2026Article
- Podometrics as a translational platform for kidney health and disease: Oshima award address 2025.Clinical and experimental nephrology · 2026Review
- Podocyte specific knockout (KO) of the natriuretic peptide clearance receptor (NPRC) attenuates diabetic kidney disease (DKD).Physiological reports · 2026Article
- Preterm Birth Increases Susceptibility to Hyperglycemia-Induced Kidney Injury With Sex-Specific Differences in Structural and Molecular Responses.Endocrinology, diabetes & metabolism · 2026Article
- Sodium-glucose cotransporter 2 inhibitor ameliorates podocyte hypertrophic stress by suppressing the mTOR/p70S6K/cyclin D1 signaling pathway in obesity-related nephropathy.Scientific reports · 2026Article
- Quantifying Podocyte Depletion and Loss in IgA Nephropathy: A Dual Tissue and Urine Podometric Approach.Kidney360 · 2026Article
- Association of Podometric Parameters with the Oxford MEST-C Score and Pretreatment eGFR Slope in Patients with IgA Nephropathy.Kidney360 · 2026Article
- Visceral Adipose Tissue Alters Podometrics and Renal Compensation After Uninephrectomy.Kidney international reports · 2026Article
- Hypertension and age-related focal global glomerulosclerosis are associated with biomarkers for cellular senescence.Physiological reports · 2026Article
- Volumetric mechanoplasticity couples melanoma drug tolerance to susceptibility to CD8bioRxiv : the preprint server for biology · 2026Article
- Biopsy Morphometrics as Predictors of Treatment Response in Primary Nephrotic Syndrome.Kidney medicine · 2026Article
- CCL5 paradoxically regulates glomerular injury by skewing macrophage polarization.JCI insight · 2025Article
- Decreased parietal epithelial cell density is linked to podocyte depletion and predictors of kidney disease progression in human kidneys.American journal of physiology. Renal physiology · 2025Article
- Lower Podocyte Number per Glomerulus Associates With Progressive CKD.Kidney international reports · 2025Article
- Reduced nephrin tyrosine phosphorylation impairs podocyte force transmission and accelerates detachment in disease.iScience · 2025Article
- Preterm birth increases susceptibility to hyperglycemia induced glomerular alterations in male mice.Scientific reports · 2025Article
- Continuous increase in podocyte numbers in the first 36 months of life-insights from forensic autopsies in Japanese children.Pediatric nephrology (Berlin, Germany) · 2025Article
- Effects of Prothrombin on Podocytopathy and Proteinuria in Glomerular Disease.Journal of the American Society of Nephrology : JASN · 2025Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The reduction in podocyte density to levels below a threshold value drives glomerulosclerosis and progression to ESRD. However, technical demands prohibit high-throughput application of conventional morphometry for estimating podocyte density. We evaluated a method for estimating podocyte density using single paraffin-embedded formalin-fixed sections. Podocyte nuclei were imaged using indirect immunofluorescence detection of antibodies against Wilms' tumor-1 or transducin-like enhancer of split 4. To account for the large size of podocyte nuclei in relation to section thickness, we derived a correction factor given by the equation CF=1/(D/T+1), where T is the tissue section thickness and D is the mean caliper diameter of podocyte nuclei. Normal values for D were directly measured in thick tissue sections and in 3- to 5-μm sections using calibrated imaging software. D values were larger for human podocyte nuclei than for rat or mouse nuclei (P<0.01). In addition, D did not vary significantly between human kidney biopsies at the time of transplantation, 3-6 months after transplantation, or with podocyte depletion associated with transplant glomerulopathy. In rat models, D values also did not vary with podocyte depletion, but increased approximately 10% with old age and in postnephrectomy kidney hypertrophy. A spreadsheet with embedded formulas was created to facilitate individualized podocyte density estimation upon input of measured values. The correction factor method was validated by comparison with other methods, and provided data comparable with prior data for normal human kidney transplant donors. This method for estimating podocyte density is applicable to high-throughput laboratory and clinical use.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.