ArticleMethods in molecular biology (Clifton, N.J.)2014
Visualization of DNA and protein-DNA complexes with atomic force microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- RNA-DNA Fusomer Fibers With Customizable Physicochemical, Mechanical, and Biological Properties for Next-Generation Therapeutics.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- The Influence of Ionic Environment on Nucleosome-Mica Interactions Revealed via Molecular Dynamics Simulations.The journal of physical chemistry. B · 2024Article
- The Influence of Ionic Environment on Nucleosome-Mica Interactions Revealed via Molecular Dynamics Simulations.bioRxiv : the preprint server for biology · 2024Article
- Dominant Analytical Techniques in DNA Nanotechnology for Various Applications.Analytical chemistry · 2024Article
- Article
- Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- 3-Aminopropylsilatrane and Its Derivatives: A Variety of Applications.Molecules (Basel, Switzerland) · 2022Review
- The International Society of RNA Nanotechnology and Nanomedicine (ISRNN): The Present and Future of the Burgeoning Field.ACS nano · 2021Article
- Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes.FEBS open bio · 2021Article
- Engineered Sumoylation-Deficient Prdx6 Mutant Protein-Loaded Nanoparticles Provide Increased Cellular Defense and Prevent Lens Opacity.Antioxidants (Basel, Switzerland) · 2021Article
- Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches.Nucleic acids research · 2021Review
- Atomic Force Microscopy Investigation of the Interactions between the MCM Helicase and DNA.Materials (Basel, Switzerland) · 2021Article
- First Step Towards Larger DNA-Based Assemblies of Fluorescent Silver Nanoclusters: Template Design and Detailed Characterization of Optical Properties.Nanomaterials (Basel, Switzerland) · 2019Article
- Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging.Journal of visualized experiments : JoVE · 2019Article
- Evaluation of Three Peptide Immobilization Techniques on a QCM Surface Related to Acetaldehyde Responses in the Gas Phase.Sensors (Basel, Switzerland) · 2018Article
- TheFrontiers in cellular and infection microbiology · 2018Article
- Accurate nanoscale flexibility measurement of DNA and DNA-protein complexes by atomic force microscopy in liquid.Nanoscale · 2017Article
- Single molecule detection of PARP1 and PARP2 interaction with DNA strand breaks and their poly(ADP-ribosyl)ation using high-resolution AFM imaging.Nucleic acids research · 2016Article
- DNA studies using atomic force microscopy: capabilities for measurement of short DNA fragments.Frontiers in molecular biosciences · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
This article describes sample preparation techniques for AFM imaging of DNA and protein-DNA complexes. The approach is based on chemical functionalization of the mica surface with aminopropyl silatrane (APS) to yield an APS-mica surface. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in the absence of divalent cations, and in a broad range of pH. The chapter describes the methodologies for the preparation of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein-DNA complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.