ArticleHistochemistry and cell biology2014
Chitosan nanoparticles are efficient carriers for delivering biodegradable drugs to neuronal cells.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Histochemistry for Molecular Imaging in Nanomedicine.International journal of molecular sciences · 2024Review
- Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cytotoxicity and Genotoxicity of Azobenzene-Based Polymeric Nanocarriers for Phototriggered Drug Release and Biomedical Applications.Polymers · 2022Article
- Histochemistry for nanomedicine: Novelty in tradition.European journal of histochemistry : EJH · 2021Article
- Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures.International journal of molecular sciences · 2021Review
- Imaging techniques in nanomedical research.European journal of histochemistry : EJH · 2020Article
- Embedding cell monolayers to investigate nanoparticle-plasmalemma interactions at transmission electron microscopy.European journal of histochemistry : EJH · 2019Article
- Carboxybetaine-modified succinylated chitosan-based beads encourage pancreatic β-cells (Min-6) to form islet-like spheroids under in vitro conditions.Journal of materials science. Materials in medicine · 2017Article
- Micellar nanocomplexes for biomagnetic delivery of intracellular proteins to dictate axon formation during neuronal development.Biomaterials · 2017Article
- Transmission electron microscopy for nanomedicine: novel applications for long-established techniques.European journal of histochemistry : EJH · 2016Review
- Enhanced Neuroprotection of Acetyl-11-Keto-β-Boswellic Acid (AKBA)-Loaded O-Carboxymethyl Chitosan Nanoparticles Through Antioxidant and Anti-Inflammatory Pathways.Molecular neurobiology · 2016Article
- The Histochemistry and Cell Biology pandect: the year 2014 in review.Histochemistry and cell biology · 2015Review
- Internalized chitosan nanoparticles persist for long time in cultured cells.European journal of histochemistry : EJH · 2015Article
- Drug delivery strategies to enhance the permeability of the blood-brain barrier for treatment of glioma.Drug design, development and therapy · 2015Review
- Engineering Nanoscale Drug Delivery Systems for Pain.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitosan nanoparticles (NPs) are biocompatible drug carriers able to cross the blood-brain barrier and represent a promising drug delivery system to the central nervous system. We used chitosan NPs to deliver the D-Ala2-D-Leu5-enkephalin (DADLE) to neuronal cells in vitro. DADLE is a hypometabolising synthetic opioid potentially useful for biomedical applications, but its short plasmatic half-life makes its in vivo administration ineffective. Here, we demonstrate by immunoelectron microscopy that (1) chitosan NPs are capable to deliver the opioid to neuronal cells; (2) DADLE is released from the internalised, opioid-loaded NPs up to 48 h; (3) in the nucleus, DADLE binds the transcription/splicing sites; (4) cells treated with DADLE-loaded NPs undergo a decrease in transcription factor amounts and proliferation rate without damage to cell organelles. In this model, chitosan NPs protected the loaded opioid from degradation, thereby prolonging its intracellular effects. These findings suggest that these NPs are efficient for the systemic and tissue administration of opioids in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.