ReviewUltramicroscopy2014
Correlative cryogenic tomography of cells using light and soft x-rays.
Review in Ultramicroscopy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Soft X-ray tomography: virtual sculptures from cell cultures.Current opinion in structural biology · 2019Review
- Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells.Emerging topics in life sciences · 2018Article
- Aberration-corrected cryoimmersion light microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Laboratory cryo x-ray microscopy for 3D cell imaging.Scientific reports · 2017Article
- Chromatin organization regulates viral egress dynamics.Scientific reports · 2017Article
- XTEND: Extending the depth of field in cryo soft X-ray tomography.Scientific reports · 2017Article
- Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.Biology of the cell · 2017Review
- Herpes simplex virus 1 induces egress channels through marginalized host chromatin.Scientific reports · 2016Article
- Imaging and characterizing cells using tomography.Archives of biochemistry and biophysics · 2015Review
- Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells.Journal of cell science · 2015Article
- Correlated light and electron microscopy: ultrastructure lights up!Nature methods · 2015Review
- Quantitatively imaging chromosomes by correlated cryo-fluorescence and soft x-ray tomographies.Biophysical journal · 2014Article
- A cool hybrid approach to the herpesvirus 'life' cycle.Current opinion in virology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Correlated imaging is the process of imaging a specimen with two complementary modalities, and then combining the two data sets to create a highly informative, composite view. A recent implementation of this concept has been the combination of soft x-ray tomography (SXT) with fluorescence cryogenic microscopy (FCM). SXT-FCM is used to visualize cells that are held in a near-native, cryopreserved. The resultant images are, therefore, highly representative of both the cellular architecture and molecular organization in vivo. SXT quantitatively visualizes the cell and sub-cellular structures; FCM images the spatial distribution of fluorescently labeled molecules. Here, we review the characteristics of SXT-FCM, and briefly discuss how this method compares with existing correlative imaging techniques. We also describe how the incorporation of a cryo-rotation stage into a cryogenic fluorescence microscope allows acquisition of fluorescence cryogenic tomography (FCT) data. FCT is optimally suited for correlation with SXT, since both techniques image the specimen in 3-D, potentially with similar, isotropic spatial resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.