ArticleBMC genomics2013
Quantitative and qualitative differences in celiac disease epitopes among durum wheat varieties identified through deep RNA-amplicon sequencing.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Evaluation of alpha-gliadin celiac disease epitopes in some Aegilops species containing the D genome.BMC plant biology · 2026Article
- Comparative Label-Free Proteomics Study on Celiac Disease-Active Epitopes in Common Wheat, Spelt, Durum Wheat, Emmer, and Einkorn.Journal of agricultural and food chemistry · 2024Article
- Variations in the composition and frequency of celiac disease epitopes among synthetic wheat lines.Frontiers in plant science · 2024Article
- Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops.Frontiers in plant science · 2023Article
- Genome editing of polyploid crops: prospects, achievements and bottlenecks.Transgenic research · 2021Review
- Exploring the alpha-gliadin locus: the 33-mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii.The Plant journal : for cell and molecular biology · 2021Article
- Article
- Reducing the Immunogenic Potential of Wheat Flour: Silencing of Alpha Gliadin Genes in a U.S. Wheat Cultivar.Frontiers in plant science · 2020Article
- Rapid evolution of α-gliadin gene family revealed by analyzing Gli-2 locus regions of wild emmer wheat.Functional & integrative genomics · 2019Article
- Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families.BMC plant biology · 2019Article
- Modern Approaches in the Identification and Quantification of Immunogenic Peptides in Cereals by LC-MS/MS.Frontiers in plant science · 2019Review
- Genetic and environmental factors affecting the expression of α-gliadin canonical epitopes involved in celiac disease in a wide collection of spelt (Triticum aestivum ssp. spelta) cultivars and landraces.BMC plant biology · 2018Article
- Development of Wheat With Hypoimmunogenic Gluten Obstructed by the Gene Editing Policy in Europe.Frontiers in plant science · 2018Article
- Differences in gluten protein composition between old and modern durum wheat genotypes in relation to 20th century breeding in Italy.European journal of agronomy : the journal of the European Society for Agronomy · 2017Article
- Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Plant methods · 2017Article
- New Insights into the Pathogenesis of Celiac Disease.Frontiers in medicine · 2017Review
- Why Oats Are Safe and Healthy for Celiac Disease Patients.Medical sciences (Basel, Switzerland) · 2016Review
- Improving wheat to remove coeliac epitopes but retain functionality.Journal of cereal science · 2016Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundWheat gluten is important for the industrial quality of bread wheat (Triticum aestivum L.) and durum wheat (T. turgidum L.). Gluten proteins are also the source of immunogenic peptides that can trigger a T cell reaction in celiac disease (CD) patients, leading to inflammatory responses in the small intestine. Various peptides with three major T cell epitopes involved in CD are derived from alpha-gliadin fraction of gluten. Alpha-gliadins are encoded by a large multigene family and amino acid variation in the CD epitopes is known to influence the immunogenicity of individual gene family members. Current commercial methods of gluten detection are unable to distinguish between immunogenic and non-immunogenic CD epitope variants and thus to accurately quantify the overall CD epitope load of a given wheat variety. Such quantification is indispensable for correct selection of wheat varieties with low potential to cause CD.
resultsA 454 RNA-amplicon sequencing method was developed for alpha-gliadin transcripts encompassing the three major CD epitopes and their variants. The method was used to screen developing grains on plants of 61 different durum wheat cultivars and accessions. A dedicated sequence analysis pipeline returned a total of 304 unique alpha-gliadin transcripts, corresponding to a total of 171 'unique deduced protein fragments' of alpha-gliadins. The numbers of these fragments obtained in each plant were used to calculate quantitative and quantitative differences between the CD epitopes expressed in the endosperm of these wheat plants. A few plants showed a lower fraction of CD epitope-encoding alpha-gliadin transcripts, but none were free of CD epitopes.
conclusionsThe dedicated 454 RNA-amplicon sequencing method enables 1) the grouping of wheat plants according to the genetic variation in alpha-gliadin transcripts, and 2) the screening for plants which are potentially less CD-immunogenic. The resulting alpha-gliadin sequence database will be useful as a reference in proteomics analysis regarding the immunogenic potential of mature wheat grains.
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