ArticleNucleic acids research2014
Transient kinetics measured with force steps discriminate between double-stranded DNA elongation and melting and define the reaction energetics.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics.Nucleic acids research · 2025Article
- Twist-stretch relations in nucleic acids.European biophysics journal : EBJ · 2023Article
- Mitotic phosphorylation inhibits the Golgi mannosidase MAN1A1.Cell reports · 2022Article
- Bio-Molecular Applications of Recent Developments in Optical Tweezers.Biomolecules · 2019Review
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- An AT-barrier mechanically controls DNA reannealing under tension.Nucleic acids research · 2016Article
- Unravelling the structural plasticity of stretched DNA under torsional constraint.Nature communications · 2016Article
- Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies.Nucleic acids research · 2015Article
- DNA under Force: Mechanics, Electrostatics, and Hydration.Nanomaterials (Basel, Switzerland) · 2015Review
- Equilibrium and kinetics of DNA overstretching modeled with a quartic energy landscape.Biophysical journal · 2014Article
- Invincible DNA tethers: covalent DNA anchoring for enhanced temporal and force stability in magnetic tweezers experiments.Nucleic acids research · 2014Article
- The transition mechanism of DNA overstretching: a microscopic view using molecular dynamics.Journal of the Royal Society, Interface · 2014Article
- Force-induced melting of DNA--evidence for peeling and internal melting from force spectra on short synthetic duplex sequences.Nucleic acids research · 2014Article
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4 authors.
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Abstract
Under a tension of ∼65 pN, double-stranded DNA undergoes an overstretching transition from its basic (B-form) conformation to a 1.7 times longer conformation whose nature is only recently starting to be understood. Here we provide a structural and thermodynamic characterization of the transition by recording the length transient following force steps imposed on the λ-phage DNA with different melting degrees and temperatures (10-25°C). The shortening transient following a 20-35 pN force drop from the overstretching force shows a sequence of fast shortenings of double-stranded extended (S-form) segments and pauses owing to reannealing of melted segments. The lengthening transients following a 2-35 pN stretch to the overstretching force show the kinetics of a two-state reaction and indicate that the whole 70% extension is a B-S transition that precedes and is independent of melting. The temperature dependence of the lengthening transient shows that the entropic contribution to the B-S transition is one-third of the entropy change of thermal melting, reinforcing the evidence for a double-stranded S-form that maintains a significant fraction of the interstrand bonds. The cooperativity of the unitary elongation (22 bp) is independent of temperature, suggesting that structural factors, such as the nucleic acid sequence, control the transition.
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