ArticleJournal of neurophysiology2014
The Drosophila neuropeptides PDF and sNPF have opposing electrophysiological and molecular effects on central neurons.
Article in Journal of neurophysiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Sleep regulation inFrontiers in neuroscience · 2026Review
- Article
- Synaptic targets of circadian clock neurons influence core clock parameters.Science advances · 2025Article
- Food sensing controlsScience advances · 2025Article
- Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters.bioRxiv : the preprint server for biology · 2025Article
- The Roles of Discrete Populations of Neurons Expressing Short Neuropeptide F in Sleep Induction in Drosophila melanogaster.Genes, brain, and behavior · 2025Article
- Gut-Brain Dialogues in Fruit Fly (Current drug targets · 2025Review
- Short neuropeptide F in integrated insect physiology.Journal of Zhejiang University. Science. B · 2024Review
- Circadian clock disruption promotes the degeneration of dopaminergic neurons in male Drosophila.Nature communications · 2023Article
- Female reproductive dormancy in Drosophila is regulated by DH31-producing neurons projecting into the corpus allatum.Development (Cambridge, England) · 2023Article
- Short neuropeptide F signaling regulates functioning of male reproductive system in Tenebrio molitor beetle.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Article
- Molecular and circuit mechanisms mediating circadian clock output in the Drosophila brain.The European journal of neuroscience · 2020Review
- Serotonergic Modulation of Aggression in Drosophila Involves GABAergic and Cholinergic Opposing Pathways.Current biology : CB · 2019Article
- Optogenetic activation of short neuropeptide F (sNPF) neurons induces sleep in Drosophila melanogaster.Physiology & behavior · 2019Article
- Peptidergic signaling from clock neurons regulates reproductive dormancy in Drosophila melanogaster.PLoS genetics · 2019Article
- A Symphony of Signals: Intercellular and Intracellular Signaling Mechanisms Underlying Circadian Timekeeping in Mice and Flies.International journal of molecular sciences · 2019Review
- Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner.Nature communications · 2019Article
- FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster.PLoS genetics · 2018Article
- Coordination between Differentially Regulated Circadian Clocks Generates Rhythmic Behavior.Cold Spring Harbor perspectives in biology · 2018Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neuropeptides have widespread effects on behavior, but how these molecules alter the activity of their target cells is poorly understood. We employed a new model system in Drosophila melanogaster to assess the electrophysiological and molecular effects of neuropeptides, recording in situ from larval motor neurons, which transgenically express a receptor of choice. We focused on two neuropeptides, pigment-dispersing factor (PDF) and small neuropeptide F (sNPF), which play important roles in sleep/rhythms and feeding/metabolism. PDF treatment depolarized motor neurons expressing the PDF receptor (PDFR), increasing excitability. sNPF treatment had the opposite effect, hyperpolarizing neurons expressing the sNPF receptor (sNPFR). Live optical imaging using a genetically encoded fluorescence resonance energy transfer (FRET)-based sensor for cyclic AMP (cAMP) showed that PDF induced a large increase in cAMP, whereas sNPF caused a small but significant decrease in cAMP. Coexpression of pertussis toxin or RNAi interference to disrupt the G-protein Gαo blocked the electrophysiological responses to sNPF, showing that sNPFR acts via Gαo signaling. Using a fluorescent sensor for intracellular calcium, we observed that sNPF-induced hyperpolarization blocked spontaneous waves of activity propagating along the ventral nerve cord, demonstrating that the electrical effects of sNPF can cause profound changes in natural network activity in the brain. This new model system provides a platform for mechanistic analysis of how neuropeptides can affect target cells at the electrical and molecular level, allowing for predictions of how they regulate brain circuits that control behaviors such as sleep and feeding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.