ArticleGenes & development2013
Anti-adaptors provide multiple modes for regulation of the RssB adaptor protein.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Dissecting host stress responses for predictable heterologous gene expression in E. coli.Nucleic acids research · 2026Article
- Complex interplay between gene deletions and the environment uncovers cellular roles for genes of unknown function inmSystems · 2025Article
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- BpfD Is a c-di-GMP Effector Protein Playing a Key Role for Pellicle Biosynthesis inInternational journal of molecular sciences · 2024Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- A negative feedback loop is critical for recovery of RpoS after stress in Escherichia coli.PLoS genetics · 2024Article
- Structure of phosphorylated-like RssB, the adaptor delivering σThe Journal of biological chemistry · 2023Article
- A negative feedback loop is critical for recovery of RpoS after stress inbioRxiv : the preprint server for biology · 2023Article
- The Transcription Factor CsgD Contributes to EngineeredInternational journal of molecular sciences · 2023Article
- DnaA and SspA regulation of the iraD gene of Escherichia coli: an alternative DNA damage response independent of LexA/RecA.Genetics · 2022Article
- Recent structural insights into the mechanism of ClpP protease regulation by AAA+ chaperones and small molecules.The Journal of biological chemistry · 2022Review
- Biomolecular mechanisms for signal differentiation.iScience · 2021Article
- Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.Protein science : a publication of the Protein Society · 2021Article
- Development of a single-stranded DNA-binding protein fluorescent fusion toolbox.Nucleic acids research · 2020Article
- Insight into the RssB-Mediated Recognition and Delivery of σBiomolecules · 2020Article
- Trouble is coming: Signaling pathways that regulate general stress responses in bacteria.The Journal of biological chemistry · 2019Review
- Insights to the Assembly of a Functionally Active Leptospiral ClpP1P2 Protease Complex along with Its ATPase Chaperone ClpX.ACS omega · 2019Article
- Structural basis for inhibition of a response regulator of σGenes & development · 2019Article
- ClpP Protease, a Promising Antimicrobial Target.International journal of molecular sciences · 2019Review
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
RpoS, an RNA polymerase σ factor, controls the response of Escherichia coli and related bacteria to multiple stress responses. During nonstress conditions, RpoS is rapidly degraded by ClpXP, mediated by the adaptor protein RssB, a member of the response regulator family. In response to stress, RpoS degradation ceases. Small anti-adaptor proteins--IraP, IraM, and IraD, each made under a different stress condition--block RpoS degradation. RssB mutants resistant to either IraP or IraM were isolated and analyzed in vivo and in vitro. Each of the anti-adaptors is unique in its interaction with RssB and sensitivity to RssB mutants. One class of mutants defined an RssB N-terminal region close to the phosphorylation site and critical for interaction with IraP but unnecessary for IraM and IraD function. A second class, in the RssB C-terminal PP2C-like domain, led to activation of RssB function. These mutants allowed the response regulator to act in the absence of phosphorylation but did not abolish interaction with anti-adaptors. This class of mutants is broadly resistant to the anti-adaptors and bears similarity to constitutively activated mutants found in a very different PP2C protein. The mutants provide insight into how the anti-adaptors perturb RssB response regulator function and activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.