ArticleInternational journal of molecular sciences2013
A simple strategy for development of single nucleotide polymorphisms from non-model species and its application in Panax.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 9 citations in OpenAlex.
- Phenotypic and Genotypic Variation of CultivatedPlants (Basel, Switzerland) · 2024Article
- Till 2018: a survey of biomolecular sequences in genusJournal of ginseng research · 2020Review
- Genome-Wide Variation Patterns Uncover the Origin and Selection in Cultivated Ginseng (Panax ginseng Meyer).Genome biology and evolution · 2017Article
- The impacts of polyploidy, geographic and ecological isolations on the diversification of Panax (Araliaceae).BMC plant biology · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Single nucleotide polymorphisms (SNPs) are widely employed in the studies of population genetics, molecular breeding and conservation genetics. In this study, we explored a simple route to develop SNPs from non-model species based on screening the library of single copy nuclear genes (SCNGs). Through application of this strategy in Panax, we identified 160 and 171 SNPs from P. quinquefolium and P. ginseng, respectively. Our results demonstrated that both P. ginseng and P. quinquefolium possessed a high level of nucleotide diversity. The number of haplotype per locus ranged from 1 to 12 for P. ginseng and from 1 to 9 for P. quinquefolium, respectively. The nucleotide diversity of total sites (πT) varied between 0.000 and 0.023 for P. ginseng and 0.000 and 0.035 for P. quinquefolium, respectively. These findings suggested that this approach is well suited for SNP discovery in non-model organisms and is easily employed in standard genetics laboratory studies.
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