ArticleInternational journal of molecular sciences2013
Selection of reliable reference genes for gene expression studies in the biofuel plant Jatropha curcas using real-time quantitative PCR.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 72 citations in OpenAlex.
- SBP-Box Transcription Factor JcSPL9 Regulates Both Seed Yield and Oil Content in the Biofuel Plant Jatropha curcas.Plant biotechnology journal · 2026Article
- Identification and Validation of qRT-PCR Reference Genes for AnalyzingCurrent issues in molecular biology · 2024Article
- Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses.Scientific reports · 2022Article
- Genome-Wide Identification and Expression Analysis of Cytokinin Response Regulator (RR) Genes in the Woody PlantInternational journal of molecular sciences · 2022Article
- An ortholog of the MADS-box gene SEPALLATA3 regulates stamen development in the woody plant Jatropha curcas.Planta · 2022Article
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- Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (Plants (Basel, Switzerland) · 2021Article
- Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica).Scientific reports · 2021Article
- Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (International journal of molecular sciences · 2021Article
- First genome edited poinsettias: targeted mutagenesis of flavonoid 3'-hydroxylase using CRISPR/Cas9 results in a colour shift.Plant cell, tissue and organ culture · 2021Article
- Comparative transcriptome analysis of gynoecious and monoecious inflorescences reveals regulators involved in male flower development in the woody perennial plant Jatropha curcas.Plant reproduction · 2020Article
- Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower.Molecular biology reports · 2020Article
- Flower-Specific Overproduction of Cytokinins Altered Flower Development and Sex Expression in the Perennial Woody PlantInternational journal of molecular sciences · 2020Article
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- Hybrid de novo transcriptome assembly of poinsettia (Euphorbia pulcherrima Willd. Ex Klotsch) bracts.BMC genomics · 2019Article
- Ectopic Expression ofInternational journal of molecular sciences · 2019Article
- Genome-wide screening of hexokinase gene family and functional elucidation of HXK2 response to cold stress in Jatropha curcas.Molecular biology reports · 2019Article
- miR172 Regulates both Vegetative and Reproductive Development in the Perennial Woody Plant Jatropha curcas.Plant & cell physiology · 2018Article
- Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment.International journal of molecular sciences · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Jatropha curcas is a promising renewable feedstock for biodiesel and bio-jet fuel production. To study gene expression in Jatropha in different tissues throughout development and under stress conditions, we examined a total of 11 typical candidate reference genes using real-time quantitative polymerase chain reaction (RT-qPCR) analysis, which is widely used for validating transcript levels in gene expression studies. The expression stability of these candidate reference genes was assessed across a total of 20 samples, including various tissues at vegetative and reproductive stages and under desiccation and cold stress treatments. The results obtained using software qBasePLUS showed that the top-ranked reference genes differed across the sample subsets. The combination of actin, GAPDH, and EF1α would be appropriate as a reference panel for normalizing gene expression data across samples at different developmental stages; the combination of actin, GAPDH, and TUB5 should be used as a reference panel for normalizing gene expression data across samples under various abiotic stress treatments. With regard to different developmental stages, we recommend the use of actin and TUB8 for normalization at the vegetative stage and GAPDH and EF1α for normalization at the reproductive stage. For abiotic stress treatments, we recommend the use of TUB5 and TUB8 for normalization under desiccation stress and GAPDH and actin for normalization under cold stress. These results are valuable for future research on gene expression during development or under abiotic stress in Jatropha. To our knowledge, this is the first report on the stability of reference genes in Jatropha.
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