ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2013
Efficacy of direct detection of pathogens in naturally infected mice by using a high-density PCR array.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Efficacy of Soiled Bedding Transfer for Transmission of Mouse and Rat Infections to Sentinels: A Systematic Review.PloS one · 2016Pooled it
- Implementation and Evaluation of Environment-Derived Dust-Based Environmental Health Monitoring in an Open-Top Housing Laboratory Animal Facility.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Bone marrow and splenic immune composition is unperturbed by short-term exposure to bedding from pet store mice.ImmunoHorizons · 2026Article
- Mouse Papillomavirus Outbreak in a Research Facility.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Evaluation of Direct Colony Sampling Compared with Sentinel-Free Soiled Bedding Testing for Murine Quarantine Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Improved Detection of Murine Pathogens Using Sentinel-Free Media Compared to Live Animal Sampling.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Evaluation of Sentinel-Free Soiled Bedding as a Replacement for Direct Sampling in PCR Detection of Rodent Pathogens during Quarantine.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Identification and Treatment of a Storage Mite Infestation in Rats (Rattus norvegicus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Comparison of Rodent Infectious Agent Detection by Exhaust Dust Testing and Traditional Sentinel Testing Using Quantitative Polymerase Chain Reaction.Journal of the American Association for Laboratory Animal Science : JAALAS · 2024Article
- Article
- Pathogen Prevalence Estimates and Diagnostic Methodology Trends in Laboratory Mice and Rats from 2003 to 2020.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- A Cross-sectional Survey on Rodent Environmental Health Monitoring Practices: Benchmarking, Associations, and Barriers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Infectivity and Shedding of Mouse Kidney Parvovirus After Oronasal Inoculation of C57BL/6, CD1, and NSG Mice.Comparative medicine · 2022Article
- Using Filter Media and Soiled Bedding in Disposable Individually Ventilated Cages as a Refinement to Specific Pathogen-free Mouse Health Monitoring Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Evaluation of In-cage Filter Paper as a Replacement for Sentinel Mice in the Detection of Murine Pathogens.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Detection of Lactate Dehydrogenase Elevating Virus in a Mouse Vivarium Using an Exhaust Air Dust Health Monitoring Program.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Adoption of Exhaust Air Dust Testing in SPF Rodent Facilities.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Comparing Mouse Health Monitoring Between Soiled-bedding Sentinel and Exhaust Air Dust Surveillance Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Murine adenoviruses: tools for studying adenovirus pathogenesis in a natural host.FEBS letters · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used a high-density array of real-time PCR assays for commonly reported rodent infectious agents (PRIA) to test naturally infected index mice and sentinel mice exposed by contact and soiled-bedding transfer. PRIA detected 14 pathogens--including viruses, bacteria, fur mites, pinworms, and enteric protozoa--in 97.2% of 28 pooled fecal samples, fur-perianal swabs, and oral swabs from 4 cages containing a total of 10 index mice. Among these pathogens, PRIA (like conventional health monitoring methods) failed to detect Mycoplasma pulmonis, Pasteurella pneumotropica, and Giardia spp. in all of the 9 contact and 9 soiled-bedding sentinels. PRIA demonstrated murine adenovirus and Cryptosporidium and Spironucleus spp. in contact but not soiled-bedding sentinels and detected Helicobacter and pinworms in fewer than half of the soiled-bedding sentinels. Of the 4 species of Helicobacter that species-specific PCR assays identified in index mice, only H. ganmani was found in soiled-bedding and contact sentinels. PRIA detected all of the pathogens in sentinels that were identified by conventional methods. Myobia musculi was detected by PCR in index and sentinel mice but missed by conventional parasitologic examinations. In summary, PRIA reproducibly detected diverse pathogens in heavily pooled specimens collected noninvasively from infected index mice antemortem. The inability of PRIA and conventional health monitoring methods (that is, parasitology, micro-biology, and serology) to demonstrate transmission of some pathogens to contact sentinels and the inefficient transmission of others to soiled-bedding sentinels underscores the importance of direct PCR testing to determine the pathogen status of rodents in quarantine and during routine colony surveillance.
Indexed as
Identifiers
24351765PMC3838611W339377225What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.