ArticlemAbs
Complex mixtures of antibodies generated from a single production qualitatively and quantitatively evaluated by native Orbitrap mass spectrometry.
Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Epitope Mapping of Japanese Encephalitis Virus Neutralizing Antibodies by Native Mass Spectrometry and Hydrogen/Deuterium Exchange.Biomolecules · 2024Article
- High-Resolution Native Mass Spectrometry.Chemical reviews · 2022Review
- Comparative Analysis of Antibodies and Heavily Glycosylated Macromolecular Immune Complexes by Size-Exclusion Chromatography Multi-Angle Light Scattering, Native Charge Detection Mass Spectrometry, and Mass Photometry.Analytical chemistry · 2022Article
- Relating glycoprotein structural heterogeneity to function - insights from native mass spectrometry.Current opinion in structural biology · 2019Review
- Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals.Nature communications · 2018Article
- Recent Advances in Next Generation Snakebite Antivenoms.Tropical medicine and infectious disease · 2018Review
- Structural Characterization of a Thrombin-Aptamer Complex by High Resolution Native Top-Down Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Recombinant snakebite antivenoms: A cost-competitive solution to a neglected tropical disease?PLoS neglected tropical diseases · 2017Article
- Quantitation of the Noncovalent Cellular Retinol-Binding Protein, Type 1 Complex Through Native Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
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- Defining the stoichiometry and cargo load of viral and bacterial nanoparticles by Orbitrap mass spectrometry.Journal of the American Chemical Society · 2014Article
- Detailed mass analysis of structural heterogeneity in monoclonal antibodies using native mass spectrometry.Nature protocols · 2014Article
- Characterization of Pharmaceutical IgG and Biosimilars Using Miniaturized Platforms and LC-MS/MS.Current pharmaceutical biotechnologyReview
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Composite antibody mixtures designed to combat diseases present a new, rapidly emerging technology in the field of biopharmaceuticals. The combination of multiple antibodies can lead to increased effector response and limit the effect of escape variants that can propagate the disease. However, parallel development of analytical technologies is required to provide fast, thorough, accurate, and robust characterization of these mixtures. Here, we evaluate the utility of native mass spectrometry on an Orbitrap platform with high mass resolving power to characterize composite mixtures of up to 15 separate antibodies. With this technique, unambiguous identification of each antibody in the mixtures was achieved. Mass measurements of the intact antibodies varied 7 ppm on average, allowing highly reproducible identification and quantitation of each compound in these complex mixtures. We show that with the high mass-resolving power and robustness of this technology, high-resolution native mass spectrometry can be used efficiently even for batch-to batch characterization.
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