ArticleBMC biology2013
Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398.
Article in BMC biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 81 citations in OpenAlex.
- Cu-miRNA-mediated redox homeostasis explains the differences in cold tolerance between AAA and ABB banana varieties.Journal of advanced research · 2026Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Identification of Cold Tolerance Transcriptional Regulatory Genes in Seedlings ofInternational journal of molecular sciences · 2024Article
- Comparative transcriptomic profiles of Paulownia catalpifolia under different degrees of chilling stress during the seedling stage.BMC genomics · 2024Article
- Effects of differentially expressed microRNAs induced by rootstocks and silicon on improving chilling tolerance of cucumber seedlings (Cucumis sativus L.).BMC genomics · 2023Article
- Combined lncRNA and mRNA Expression Profiles Identified the lncRNA-miRNA-mRNA Modules Regulating the Cold Stress Response inInternational journal of molecular sciences · 2023Article
- PotatoInternational journal of molecular sciences · 2023Article
- MicroRNA398: A Master Regulator of Plant Development and Stress Responses.International journal of molecular sciences · 2022Review
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Article
- The role of VvMYBA2r and VvMYBA2w alleles of the MYBA2 locus in the regulation of anthocyanin biosynthesis for molecular breeding of grape (Vitis spp.) skin coloration.Plant biotechnology journal · 2021Article
- Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress.Plant cell reports · 2021Article
- Transcriptome and Metabolome Analyses of the Flowers and Leaves ofFrontiers in genetics · 2021Article
- BrrICE1.1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of turnip (Brassica rapa var. rapa).BMC plant biology · 2020Article
- Overexpression of ArabidopsisPlant signaling & behavior · 2020Article
- The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance inInternational journal of molecular sciences · 2019Article
- An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development.BMC plant biology · 2019Article
- The Heterologous Expression of a Chrysanthemum nankingense TCP Transcription Factor Blocks Cell Division in Yeast and Arabidopsis thaliana.International journal of molecular sciences · 2019Article
- Transcriptome-wide identification of miRNA targets and a TAS3-homologous gene in Populus by degradome sequencing.Genes & genomics · 2019Article
- Characterization and Cloning of Grape Circular RNAs Identified the Cold Resistance-RelatedPlant physiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundICE (Inducer of CBF Expression) family genes play an important role in the regulation of cold tolerance pathways. In an earlier study, we isolated the gene CdICE1 from Chrysanthemum dichrum and demonstrated that freezing tolerance was enhanced by CdICE1 overexpression. Therefore, we sought to determine the mechanism by which ICE1 family genes participate in freezing tolerance.
resultsUsing EMSA (Electrophoretic Mobility Shift Assay) and yeast one-hybrid assays, we confirmed that CdICE1 binds specifically to the MYC element in the CdDREBa promoter and activates transcription. In addition, overexpression of CdICE1 enhanced Arabidopsis freezing tolerance after transition from 23°C to 4°C or 16°C. We found that after acclimation to 4°C, CdICE1, like Arabidopsis AtICE1, promoted expression of CBFs (CRT/DRE Binding Factor) and their genes downstream involved in freezing tolerance, including COR15a (Cold-Regulated 15a), COR6.6, and RD29a (Responsive to Dessication 29a). Interestingly, we observed that CdICE1-overexpressing plants experienced significant reduction in miR398. In addition, its target genes CSD1 (Copper/zinc Superoxide Dismutase 1) and CSD2 showed inducible expression under acclimation at 16°C, indicating that the miR398-CSD pathway was involved in the induction of freezing tolerance.
conclusionsOur data indicate that CdICE1-mediated freezing tolerance occurs via different pathways, involving either CBF or miR398, under acclimation at two different temperatures.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.