ArticleMolecular microbiology2014
Identification of functionally important conserved trans-membrane residues of bacterial PIB -type ATPases.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 12 citations in OpenAlex.
- Characterization of three predicted zinc exporters inFrontiers in veterinary science · 2023Article
- Structure and ion-release mechanism of PeLife · 2021Article
- Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.Journal of experimental botany · 2019Article
- The highly synergistic, broad spectrum, antibacterial activity of organic acids and transition metals.Scientific reports · 2017Article
- L-glutamine Induces Expression of Listeria monocytogenes Virulence Genes.PLoS pathogens · 2017Article
- Zinc Resistance Mechanisms of P1B-type ATPases in Sinorhizobium meliloti CCNWSX0020.Scientific reports · 2016Article
- The uncoupled ATPase activity of the ABC transporter BtuC2D2 leads to a hysteretic conformational change, conformational memory, and improved activity.Scientific reports · 2016Article
- Article
- Diversity of the metal-transporting P1B-type ATPases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Powered by ATP hydrolysis, P(IB) -ATPases drive the energetically uphill transport of transition metals. These high affinity pumps are essential for heavy metal detoxification and delivery of metal cofactors to specific cellular compartments. Amino acid sequence alignment of the trans-membrane (TM) helices of P(IB)-ATPases reveals a high degree of conservation, with ∼ 60-70 fully conserved positions. Of these conserved positions, 6-7 were previously identified to be important for transport. However, the functional importance of the majority of the conserved TM residues remains unclear. To investigate the role of conserved TM residues of P(IB)-ATPases we conducted an extensive mutagenesis study of a Zn(2+)/Cd(2+) P(IB)-ATPase from Rhizobium radiobacter (rrZntA) and seven other P(IB)-ATPases. Of the 38 conserved positions tested, 24 had small effects on metal tolerance. Fourteen mutations compromised in vivo metal tolerance and in vitro metal-stimulated ATPase activity. Based on structural modelling, the functionally important residues line a constricted 'channel', tightly surrounded by the residues that were found to be inconsequential for function. We tentatively propose that the distribution of the mutable and immutable residues marks a possible trans-membrane metal translocation pathway. In addition, by substituting six trans-membrane amino acids of rrZntA we changed the in vivo metal specificity of this pump from Zn(2+)/Cd(2+) to Ag(+).
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